Materials and methods for controlling oxidation and reduction in biosynthetic pathways of species of the genera ralstonia and cupriavidus and organisms related thereto
Methods for increasing carbon-based chemical product yield in an organism by perturbing redox balance in an organism as well as nonnaturally occurring organisms with perturbed redox balance and methods for their use in producing carbon-based chemical products are provided.
1. A method for increasing yield of 3-hydroxybutyrate in a Cupriavidus or Ralstonia organism, said method consisting of perturbing redox balance in said Cupriavidus or Ralstonia organism by knocking out at least a portion of its phaC1AB1 gene locus to diminish polyhydroxybutyrate synthesis as compared to an unperturbed wild-type Cupriavidus or Ralstonia organism and by modifying the organism to overexpress an endogenous or to express an exogenous nucleic acid sequence encoding a transhydrogenase enzyme selected from EC 1.6.1.1, EC 1.6.1.2, or EC 7.1.1.1 and/or deleting a dehydrogenase, culturing said Cupriavidus or Ralstonia organism under conditions to produce 3-hydroxybutyrate, and recovering 3-hydroxybutyrate from the organism, thereby increasing 3-hydroxybutyrate yield as compared to Cupriavidus or Ralstonia organism without the modifications.
2. The method of claim 1 wherein the organism overexpresses a native or expresses an exogenous transhydrogenase enzyme selected from EC 1.6.1.1, EC 1.6.1.2, or EC 7.1.1.1.
3. The method of claim 1 wherein phosphate, carbon, nitrogen, and/or oxygen are limited.
4. A method for producing 3-hydroxybutyrate in an organism, said method consisting of fermenting a non-naturally occurring organism selected from a species of Cupriavidus or Ralstonia with at least a portion of its phaC1AB1 gene locus knocked out to diminish polyhydroxybutyrate synthesis as compared to an unperturbed wild-type Cupriavidus or Ralstonia organism and modified to perturb redox balance by modifying the organism to overexpress an endogenous or to express an exogenous nucleic acid sequence encoding a transhydrogenase enzyme selected from EC 1.6.1.1, EC 1.6.1.2, or EC 7.1.1.1 and/or deleting a dehydrogenase enzyme with a carbon source; culturing said Cupriavidus or Ralstonia under conditions to produce 3-hydroxybutyrate and recovering 3-hydroxybutyrate from the organism.
5. The method of claim 4 wherein perturbing redox balance in the organism increases 3-hydroxybutyrate yield in the organism as compared to an organism without the modifications.
6. The method of claim 4 wherein the nonnaturally occurring organism overexpresses a native or expresses an exogenous transhydrogenase enzyme_selected from EC 1.6.1.1, EC 1.6.1.2, or EC 7.1.1.1.
7. The method of claim 4 wherein the carbon source is derived from a biological or nonbiological feedstock.
8. The method of claim 7 wherein feedstock fed to the fermentation process comprises a gaseous or liquid stream.