Human alpha-galactosidase variants
The present invention provides engineered human alpha-galactosidase polypeptides and compositions thereof. The engineered human alpha-galactosidase polypeptides have been optimized to provide improved thermostability, serum stability, improved cellular uptake, stability under both acidic (pH<4) and basic (pH>7) conditions, reduced immunogenicity, and improved globotriaosylceramide removal from cells. The invention also relates to the use of the compositions comprising the engineered human alpha-galactosidase polypeptides for therapeutic purposes.
1. A recombinant alpha galactosidase A comprising an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 158, and wherein the amino acid sequence of said recombinant alpha galactosidase A comprises glycine at the position corresponding to residue 333 of SEQ ID NO: 158.
2. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A further comprises at least one additional substitution or substitution set at one or more of positions 24/202, 39/47, 39/47/217, 39/151, 39/282/337/398, 39/337/343/398, 39/393/398, 47/130, 47/151, 47/343/345/393, 48, 48/68, 48/68/217/391/393, 48/68, 48/217, 48/345/393, 48/393, 59/143, 68, 68/345, 130, 130/158, 130/158/393, 130/345/393, 143, 143/271, 143/387, 151, 151/158/217/343/345/393, 151/206/282/337/343/345/398, 151/282/393, 151/345/393/398, 151/393, 158, 158/393, 202, 206, 206/217, 217, 217/337/345/398, 271, 282/393, 337/343/345/398, 343, 343/345/393/398, 345, 393, or 393/398, wherein the amino acid position(s) correspond to the amino acid sequence of SEQ ID NO: 158.
3. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A comprises the amino acid sequence of SEQ ID NO: 240, 276, 288, or 292.
4. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more thermostable than the alpha galactosidase A of SEQ ID NO: 2.
5. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more stable at pH 7 than the alpha galactosidase A of SEQ ID NO: 2.
6. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more stable at pH 4 than the alpha galactosidase A of SEQ ID NO: 2.
7. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more stable to exposure to serum than the alpha galactosidase A of SEQ ID NO: 2.
8. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more lysosomally stable than the alpha galactosidase A of SEQ ID NO: 2.
9. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is more readily taken up by cells than the alpha galactosidase A of SEQ ID NO: 2.
10. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A depletes more globotriaosylceramide from cells than the alpha galactosidase A of SEQ ID NO: 2.
11. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A is purified.
12. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A exhibits at least one property selected from:
i) enhanced catalytic activity as compared to the alpha galactosidase A of SEQ ID NO: 2;
ii) increased tolerance to pH 7 as compared to the alpha galactosidase A of SEQ ID NO: 2;
iii) increased tolerance to pH 4 as compared to the alpha galactosidase A of SEQ ID NO: 2;
iv) increased tolerance to serum as compared to the alpha galactosidase A of SEQ ID NO: 2;
v) increased uptake into cells as compared to the alpha galactosidase A of SEQ ID NO: 2;
vi) increased depletion of globotriaosylceramide from cells as compared to the alpha galactosidase A of SEQ ID NO: 2;
vii) reduced immunogenicity as compared to the alpha galactosidase A of SEQ ID NO: 2;
or a combination of any of i), ii), iii), iv), v), vi), and/or vii).
13. A composition comprising at least one recombinant alpha galactosidase A of claim 1 .
14. A pharmaceutical composition comprising at least one recombinant alpha galactosidase A of claim 1 .
15. The pharmaceutical composition of claim 14 , further comprising a pharmaceutically acceptable carrier and/or excipient.
16. The pharmaceutical composition of claim 14 , wherein said composition is suitable for parenteral injection or infusion to a human.
17. A method for treating the symptoms of Fabry disease in a subject, comprising administering the pharmaceutical composition of claim 14 to a subject having Fabry disease.
18. The method of claim 17 , wherein said symptoms of Fabry disease are ameliorated.
19. The method of claim 17 , wherein said subject is able to eat a diet that is less restricted in its fat content than diets required by subjects exhibiting the symptoms of Fabry disease.
20. The method of claim 17 , wherein said subject is an infant or child.
21. The method of claim 17 , wherein said subject is an adult or young adult.
22. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase A further comprises at least one additional substitution or substitution set 24S/202N, 39V/47D, 39V/47V/217K, 39V/151L, 39V/282N/337R/398A, 39V/337R/343G/398A, 39V/393V/398A, 47V/130E, ATV/I51L, 47V/343D/345Q/393V, 48D, 48D/68E, 48D/217K, 48D/345Q/393V, 48D/393V, 59A/143S, 68E, 68E/345Q, 130E, 130E/158R, 130E/158R/393V, 130E/345Q/393V, 143S/271N, 143S/387N, 151L, 151L/158R/217K/343G/345Q/393V, 151L/206S/282N/337R/343D/345Q/398A, 151L/282N/393V, 151L/345Q/393V/398A, 151L/393V, 158R, 158R/393V, 202N, 2068S, 2068/217K, 217K, 217K/337R/345Q/398A, 271N, 282N/393V, 337R/343G/345Q/398A, 343D, 343D/345Q/393V/398A, 393V, or 393V/398A, wherein the amino acid position(s) correspond to the amino acid sequence of SEQ ID NO: 158.
23. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase comprises an amino acid sequence that has at least 91%, sequence identity to the amino acid sequence of SEQ ID NO: 158.
24. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase comprises an amino acid sequence that has at least 95%, sequence identity to the amino acid sequence of SEQ ID NO: 158.
25. The recombinant alpha galactosidase A of claim 1 , wherein said recombinant alpha galactosidase comprises an amino acid sequence that has at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 158.