Plant desaturases compositions and uses
By this invention, compositions and methods of use of plant desaturase enzymes, especially Δ-9 desaturases, are provided. Of special interest are methods and compositions of amino acids and nucleic acid sequences related to biologically active plant desaturases as well as sequences, especially nucleic acid sequences, which are to be used as probes, vectors for transformation or cloning intermediates. Biologically active sequences may be found in a sense or anti-sense orientation as to transcriptional regulatory regions found in various constructs.
1. A method of modifying the fatty acid composition of a plant host cell from a given weight percentage of saturated fatty acids to a different weight percentage of saturated fatty acids, said method comprising:
growing a host plant cell having a recombinant DNA construct integrated into the genome of said cell or a parent cell thereof, said construct comprising a nucleotide sequence encoding a plant Δ-9 desaturase protein having any one of the amino acid peptide sequences shown in SEQ ID NOS 1–7 and SEQ ID NOS 9–11 under the control of regulatory elements functional in said plant cell during lipid accumulation under conditions which will promote the activity of said regulatory elements.
2. The method of claim 1 wherein said regulatory elements function preferentially in plant seed cells.
3. The method of claim 1 wherein said plant host cell is selected from the group consisting of rapeseed, sunflower, castor, cotton, Cuphea , peanut, soybean, oil palm and corn.
4. The method of claim 1 , wherein said plant host cell is a Brassica cell.
5. The method of claim 1 , wherein said plant host is a Brassica cell and wherein said construct encodes a Brassica Δ-9-desaturase in an antisense orientation with respect to said regulatory elements.
6. The method of claim 1 , wherein said host cell is from an oil producing plant.
7. The method of claim 1 ,
wherein at least one of said plant Δ-9 desaturase and said regulatory elements is heterologous to said plant host cell.
8. The method of claim 1 ,
wherein said nucleotide sequence is in an antisense orientation with respect to said regulatory elements.
9. The method of claim 1 ,
wherein said regulatory elements preferentially are functional in plant seed.
10. A method of modifying the fatty acid composition of oil triglycerides in an oil producing plant host cell from a given weight percentage of saturated fatty acids to a difference weight percentage of saturated fatty acids comprising:
growing a host plant cell having a recombinant DNA construct integrated into the genome of said cell or parent cell thereof, said construct comprising a nucleotide sequence encoding a plant Δ-9 desaturase protein having any one of the amino acid peptide sequences shown as SEQ ID NOS 1–7 and SEQ ID NOS 9–11 under the control of regulatory elements functional in said plant cell during lipid accumulation under conditions which will promote the activity of said regulatory elements.
11. The method of claim 10 wherein said regulatory elements function preferentially in plant seed cells.
12. The method of claim 10 wherein said plant host cell is selected from the group consisting of rapeseed, sunflower, castor, cotton, Cuphea , peanut, soybean, oil palm and corn.
13. The method of claim 10 , wherein said plant host cell is a Brassica cell.
14. The method of claim 10 , wherein said plant host is a Brassica cell and wherein said construct encodes a Brassica Δ-9-desaturase in an antisense orientation with respect to said regulatory elements.
15. The method according to claim 10 , wherein at least one of said plant Δ-9 desaturase and said regulatory elements is heterologous to said plant host cell.
16. The method of claim 10 ,
wherein said nucleotide sequence is in an antisense orientation with respect to regulatory elements.
17. The method according to claim 7 or 10 , wherein said nucleotide sequence encodes the amino acid peptide KEIPDDYFVVLVGMITEEALPTYQTMLNT (amino acids 23–52 of SEQ ID NO: 2).
18. The method according to claim 1 or 10 , wherein said nucleotide sequence encodes the amino acid peptide DYADILEFLVGRWK (SEQ ID NO: 10).
19. The method according to claim 1 or 10 , wherein said nucleotide sequence is SEQ ID NO:12.
20. The method according to claim 1 or 10 , wherein said nucleotide sequence encodes the mature plant desaturase protein having the amino acid sequence in SEQ ID NO: 13.
21. The method according to claim 1 or 10 , wherein said nucleotide sequence is SEQ ID NO: 15.
22. The method according to claim 1 or 10 , wherein said nucleotide sequence encodes the plant desaturase protein having the amino acid sequence in SEQ ID NO: 16.
23. The method according to claim 1 or 10 , wherein said nucleotide sequence is SEQ ID NO: 19.
24. The method accord to claim 1 or 10 , wherein said nucleotide sequence encodes the plant desaturase protein having the amino acid sequence in SEQ ID NO: 20.
25. The method according to claim 1 or 10 , wherein said Δ-9 desaturase protein has the amino acid sequence shown in SEQ ID NO: 20.
26. The method according to claim 25 , wherein said amino acid sequence is encoded by the DNA sequence shown in SEQ ID NO:19.