Compositions for the electronic detection of analytes utilizing monolayers
The present invention relates to the use of self-assembled monolayers with mixtures of conductive oligomers and insulators to detect target analytes.
1. A composition comprising:
a) an electrode comprising:
i) a monolayer comprising insulators; and
ii) a capture binding ligand wherein said capture binding ligand is covalently attached to said electrode via an attachment linker; and,
b) a target analyte binding ligand comprising at least one non-electrochemiluminescent electron transfer moiety (ETM) covalently attached to said target analyte binding ligand.
2. A composition according to claim 1 wherein said attachment linker is selected from the group consisting of conductive oligomers and insulators.
3. A composition according to claim 1 wherein said capture binding ligand is a nucleic acid.
4. A composition according to claim 1 wherein said capture binding ligand is a protein.
5. A composition according to claim 1 wherein said ETM is a metallocene.
6. A composition according to claim 5 wherein said metallocene is ferrocene.
7. A composition according to claim 1 wherein said insulators are alkyl chains.
8. A composition comprising:
a) an electrode comprising:
i) a monolayer comprising insulators; and
ii) a capture binding ligand wherein said capture binding ligand is covalently attached to said electrode via an attachment linker; and,
b) a target analyte binding ligand comprising:
i) nucleic acid;
ii) at least one non-electrochemiluminescent electron transfer moiety (ETM) covalently attached to said target analyte binding ligand.
9. A composition according to claim 8 wherein said attachment linker is selected from the group consisting of conductive oligomers and insulators.
10. A composition according to claim 8 wherein said capture binding ligand is a nucleic acid.
11. A composition according to claim 8 wherein said capture binding ligand is a protein.
12. A composition according to claim 8 wherein said ETM is a metallocene.
13. A composition according to claim 12 wherein said metallocene is ferrocene.
14. A composition according to claim 8 wherein said insulators are alkyl chains.
15. A composition according to claim 1 wherein said monolayer further comprises conductive oligomers.
16. A composition according to claim 8 wherein said monolayer further comprises conductive oligomers.
17. A composition according to claim 2 wherein said attachment linker is a conductive oligomer.
18. A composition according to claim 2 wherein said attachment linker is an insulator.
19. A composition according to claim 9 wherein said attachment linker is a conductive oligomer.
20. A composition according to claim 9 wherein said attachment liker is an insulator.