Nuclear targeting by means of bacterial proteins
A nuclear delivery construct comprises (i) protein H or a fragment or derivative thereof that is capable of being targeted to the nucleus of a eukaryotic cell; and associated therewith (ii) one or more other components whose targeting to the nucleus of the eukaryotic cell is desired.
1. A method of targeting one or more components to the nucleus of a lymphocyte or epithelial cell, said method comprising administering a nuclear delivery construct to said lymphocyte or epithelial cell, wherein said nuclear delivery construct is comprised of:
(i) a polypeptide comprising the sequence as set forth in SEQ ID NO: 6, amino acids 42 to 376 of SEQ ID NO: 6, amino acids 42 to 346 of SEQ ID NO: 6 or amino acids 42 to 326 of SEQ ID NO: 6; and conjugated or fused therewith;
(ii) said one or more components whose targeting to the nucleus of the lymphocyte or epithelial cell is desired.
2. The method of claim 1 , wherein the construct is targeted to the nucleus of a cell in which nucleophosmin (NPM)/B23 is up-regulated.
3. The method of claim 1 , wherein component (i) of said construct interacts with actin, NPM/B23, protein SET or hnRNP A2/B1.
4. The method of claim 2 , wherein component (i) of said construct interacts with actin, NPM/B23, protein SET or hnRNP A2/B 1.
5. The method of claim 1 , wherein the cell is a tumor cell or proliferating cell.
6. A method of targeting one or more components to the nucleus of a lymphocyte, said method comprising administering a nuclear delivery construct to said lymphocyte, wherein said nuclear delivery construct is comprised of:
(i) a polypeptide comprising the sequence as set forth in SEQ ID NO: 6, amino acids 42 to 376 of SEQ ID NO: 6, amino acids 42 to 346 of SEQ ID NO: 6 or amino acids 42 to 326 of SEQ ID NO: 6; and conjugated or fused therewith;
(ii) said one or more components whose targeting to the nucleus of the lymphocyte is desired.
7. A method of targeting one or more components to the nucleus of a lymphocyte, said method comprising administering a nuclear delivery construct to said lymphocyte, wherein said nuclear delivery construct is comprised of:
(i) a polypeptide comprising the sequence as set forth in SEQ ID NO: 6, amino acids 42 to 376 of SEQ ID NO: 6, amino acids 42 to 346 of SEQ ID NO: 6 or amino acids 42 to 326 of SEQ ID NO: 6; and covalently associated therewith;
(ii) said one or more components whose targeting to the nucleus of the lymphocyte is desired.
8. A method of targeting a single component to the nucleus of a lymphocyte, said method comprising administering a nuclear delivery construct to said lymphocyte, wherein said nuclear delivery construct is comprised of:
(i) a polypeptide comprising the sequence as set forth in SEQ ID NO: 6, amino acids 42 to 376 of SEQ ID NO: 6, amino acids 42 to 346 of SEQ ID NO: 6 or amino acids 42 to 326 of SEQ ID NO: 6; and covalently associated therewith;
(ii) the component whose targeting to the nucleus of the lymphocyte cell is desired.