Diagnosis of disease state using mRNA profiles in peripheral leukocytes
Disclosed are diagnostic techniques for the detection of human disease states that affect gene expression in peripheral leukocytes. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in the peripheral blood of individuals with such a disease state compared to normal healthy individuals. The invention further relates to methods for detection of protein species that are differentially expressed in the peripheral blood of individuals with such a disease state compared to normal healthy individuals. Genetic probes, antibody probes and methods useful in monitoring the progression and diagnosis of two specific disease states, prostatic cancer and breast cancer, are described.
1. A method of detecting metastatic cancer, comprising the steps of:
(a) detecting the quantity of a disease marker mRNA expressed in human peripheral blood; and
(b) comparing the quantity of said marker to the quantity expressed in peripheral blood of a normal individual;
wherein said mRNA comprises SEQ ID NO:49 and wherein a difference in quantity of expression is indicative of metastatic cancer.
2. The method of claim 1 , further comprising the steps of
a) providing primers that selectively amplify the metastatic cancer marker;
b) amplifying said nucleic acid with said primers to form nucleic acid amplification products;
c) detecting said nucleic acid amplification products; and
d) measuring the amount of said nucleic acid amplification products formed.
3. The method of claim 1 , wherein said mRNA is amplified by an RNA polymerase reaction.
4. The method of claim 1 , wherein said mRNA is amplified by reverse transcriptase polymerase chain reaction or the ligase chain reaction.
5. The method of claim 1 , wherein said detecting is by RNA fingerprinting, branched DNA or a nuclease protection assay.
6. The method of claim 1 , wherein the metastatic cancer is metastatic prostate cancer.
7. The method of claim 1 , wherein the metastatic cancer is metastatic breast cancer.