Composition for accelerating seed germination and plant growth
View Patent ↗Lipo Chitooligosaccharide (LCO) [NodBj-V(C18:1,Mefuc)] isolated from Bradyrhizobium japonicum strain 532C was able to stimulate seed germination/seedling emergence, or in the case of potato, sprouting, of a number of crop plants representing eight distantly related plant families (Poaceae, Fabaceae, Brassicaceae, Cucurbitaceac, Malvaceae, Asteraceae, Chenopodiaceae and Solanaceae) of plants, at 25 and/or at 15° C. It also promoted sprouting potato minitubers. Other LCOs [NodRM-V(C 16:2,5 ) and LCO from R. leguminosarum ] were also shown to also display growth-promoting effects on the tested crop plants. The compositions comprising at least one LCO are shown to be effective in promoting growth under both laboratory and field conditions. The invention thus also relates to methods for promoting seed germination and/or seedling emergence and/or growth of plants comprising subjecting the seeds and/or plants to an effective amount of an agricultural composition comprising at least one LCO.
1. A method for enhancing seed germination or seedling emergence of a plant crop comprising the steps of:
providing a composition that comprises an effective amount of at least one lipo chitooligosaccharide (LCO) and an agriculturally suitable carrier; and
applying the composition in the immediate vicinity of a seed or seedling in an effective amount for enhancing seed germination or seedling emergence in comparison to an untreated seed or seedling.
2. A method for enhancing in a non-legume, seed germination, seedling emergence or growth of a plant crop comprising the steps of:
providing a composition that comprises an effective amount of at least one lipo chitooligosaccharide (LCO) and an agriculturally suitable carrier; and
applying the composition in the immediate vicinity of a seed, root or plant in an effective amount for enhancing seed germination, seedling emergence or growth of said plant in comparison to an untreated plant.
3. The method according to claim 2 , wherein said plant crop is selected from the group consisting of Poaceae, Cucurbitaceae, Malvaceae, Asteraceae, Chenopodiaceae, Solanaceae and Brassicaceae.
4. The method according to claim 3 , wherein said plant crop is selected from the group consisting of corn, cotton, cucumber, cantaloupe, lettuce, beet, canola and potato.
5. The method according to claim 1 , wherein said LCO is obtainable from a rhizobia selected from the group consisting of Bradyrhizobium japonicum, Rhizobium meliloti and Rhizobium leguminosarum.
6. The method according to claim 5 , wherein said LCO is present in said composition at a concentration of between about 10 −5 M to about 10 −14 M.
7. The method according to claim 5 , wherein said LCO is present in said composition at a concentration of between about 10 −6 M to about 10 −12 M.
8. The method according to claim 5 , wherein said LCO is present in said composition at a concentration of between about 10 −7 M to about 10 −10 M.
9. The method according to claim 1 , wherein said composition is effective in enhancing seed germination or seedling emergence under field conditions.
10. The method according to claim 1 , wherein said plant crop is a member of the Fabaceae family.
11. The method according to claim 10 , wherein said plant crop is selected from the group consisting of soybean, bean, alfalfa and clover.
12. The method according to claim 10 , wherein said LCO is obtainable from a rhizobia selected from the group consisting of Bradyrhizobium japonicum, Rhizobium meliloti and Rhizobium leguminosarum.
13. The method according to claim 12 , wherein said LCO is present in said composition at a concentration of between about 10 −5 M to about 10 −14 M.
14. The method according to claim 12 , wherein said LCO is present in said composition at a concentration of between about 10 −6 M to about 10 −12 M.
15. The method according to claim 12 , wherein said LCO is present in said composition at a concentration of between about 10 −7 M to about 10 −10 M.
16. The method according to claim 10 , wherein said composition is effective in enhancing seed germination or seedling emergence under field conditions.
17. A method for breaking the dormancy or quiescence of a plant comprising the steps of:
providing an agricultural composition comprising at least one lipo chitooligosaccharide (LCO) and an agriculturally suitable carrier; and
applying the composition in the immediate vicinity of a seed, tuber or root in an effective amount to enable a breaking of the dormancy or quiescence of the seed, tuber, or root, in comparison to an untreated seed, tuber, or root.
18. The method according to claim 17 , wherein said plant is a member of the family of Solonaceae.
19. The method according to claim 18 , wherein said plant is a potato.
20. The method according to claim 19 , wherein said growth-promoting activity of said composition enables an increase in yield.
21. The method according to claim 19 , wherein said composition further comprises gibberellic acid.
22. A method for enhancing seed germination or seedling emergence of a plant crop comprising the steps of:
providing a rhizobial strain that expresses a lipo chitooligosaccharide (LCO); and
incubating the rhizobial strain in the immediate vicinity of one of a seed or seedling of said plant such that said LCO enhances seed germination or seedling emergence in comparison to a non-inoculated seed or seedling.
23. A method for enhancing in a non-legume, seed germination, seedling emergence or growth of a plant crop comprising the steps of:
providing a rhizobial strain that expresses a lipo chitooligosaccharide (LCO); and
incubating the rhizobial strain in the immediate vicinity of one of a seed or root of said plant such that said LCO enhances seed germination, seedling emergence or growth of said plant crop, wherein said incubation enhances seed germination, seedling emergence or growth in comparison to a non-inoculated seed or root of said plant.
24. The method of claim 23 , wherein said plant crop is selected from the group consisting of Poaceae, Cucurbitaceae, Malvaceae, Asteraceae, Chenopodiaceae, Solanaceae and Brassicaceae.
25. The method of claim 24 , wherein said plant crop is selected from the group consisting of corn, cotton, cucumber, cantaloupe, lettuce, beet, canola and potato.
26. The method of claim 22 , wherein said rhizobia is selected from Bradyrhizobium japonicum, Rhizobium meliloti and Rhizobium leguminosarum.
27. The method of claim 22 , wherein said LCO enhances seed germination or seedling emergence under field conditions.
28. The method of claim 22 , wherein said plant crop is a legume in the Fabaceae family and wherein said LCO enhances seed germination or seedling emergence under field conditions.
29. The method of claim 17 , wherein said composition comprises a bacterial strain which expresses said LCO.
30. The method of claim 29 , wherein said bacterial strain is a rhizobial strain.
31. The method of claim 1 , wherein said composition comprises a bacterial strain that expresses said LCO.
32. The method of claim 31 , wherein said bacterial strain is a rhizobial strain.
33. A method for enhancing seed germination or seedling emergence of a plant crop comprising the steps of:
providing a bacterial strain that expresses a lipo chitooligosaccharide (LCO); and
incubating said bacterial strain in the immediate vicinity of one of a seed or seedling of said plant such that said LCO enhances seed germination or seedling emergence of said plant crop, wherein said incubation enhances seed germination or seedling emergence in comparison to a non-inoculated seed or seedling of said plant.
34. A method for enhancing seed germination or seedling emergence of a plant crop comprising the step of:
providing a bacterial strain that expresses a lipo chitooligosaccharide (LCO) in the immediate vicinity of one of a seed or seedling of said plant such that said bacterial strain, upon expression of said LCO, enhances seed germination or seedling emergence of said plant crop, in comparison to a non-treated seed or seedling of said plant.
35. The method of claim 34 wherein said bacterial strain is a rhizobial strain.