Kolla2-desiccated avian sternal cartilage powder
Kolla2 powder compositions, method of preparing the compositions and use of the compositions in treating arthritic joint cartilage diseases. The compositions are orally administered to human in need of cartilage cell repair in a daily dietary supplement dosage of between about 2,400 mg and 3,600 mg.
1. A method of preparing cartilage-derived material comprising collagen type II in powder form, comprising the steps:
(a) cutting avian sternal cartilage to within not less than 2 mm of the sternum and removing the cut sternal cartilage from the sternum;
(b) freezing the cut sternal cartilage into frozen cartilage;
(c) grinding the frozen cartilage into ground-cartilage;
(d) suspending the ground cartilage in an aqueous solution;
(e) sterilizing the ground cartilage;
(f) filtering the sterilized ground cartilage;
(g) defatting the ground cartilage;
(h) filtering the defatted ground cartilage;
(i) drying the ground cartilage; and
(j) milling the dried ground cartilage into powder form.
2. The method of claim 1 wherein the aqueous solution is water.
3. The method of claim 1 wherein the sterilizing step includes heating the ground cartilage to at least 95° C. for at least 30 minutes.
4. The method of claim 1 wherein the defatting step includes treating the ground cartilage with ethanol.
5. The method of claim 1 , wherein the drying step includes heating the ground cartilage to at least 95° C. for at least 6 hours.
6. The method of claim 1 , wherein the dried ground cartilage is milled into a powder form having a size between about 80 mesh to 200 mesh.