Regulation of immune responses by attractin
The invention features attracting polypeptides and nucleic acids encoding them. The attractin polypeptides are useful for enhancing immune responses.
1. An isolated DNA comprising a nucleic acid sequence that encodes an amino acid sequence comprising SEQ ID NO:12.
2. An isolated DNA comprising a nucleic acid sequence consisting of SEQ ID NO:13.
3. An isolated nucleic acid comprising a nucleic acid sequence encoding a fusion protein, the fusion protein consisting of:
(a) SEQ ID NO:12 or an antigenic fragment of SEQ ID NO:12, wherein the antigenic fragment is at least five amino acids long; and
(b) a heterologous polypeptide.
4. A vector comprising the isolated DNA of claim 1 .
5. The vector of claim 4 , wherein the nucleic acid sequence is operably linked to a regulatory element that allows expression of said nucleic acid sequence in a cell.
6. An isolated cell comprising the vector of claim 5 .
7. A method of producing a polypeptide, the method comprising culturing the cell of claim 6 and purifying the polypeptide from the cell.
8. A vector comprising the isolated nucleic acid of claim 3 .
9. The vector of claim 8 , wherein the nucleic acid is operably linked to a regulatory element that allows expression of said nucleic acid in a cell.
10. An isolated cell comprising the vector of claim 8 .
11. A method of producing a fusion protein, the method comprising culturing the cell of claim 10 and purifying the fusion protein from the cell.
12. An isolated DNA comprising:
(a) a nucleic acid sequence that (i) encodes a polypeptide that enhances spreading of a macrophage or a monocyte and (ii) hybridizes to the complement of SEQ ID NO:13 under the following conditions: hybridization in 6× SSC at 30° C., followed by one or more washes in 0.2× SSC and 0.1% sodium dodecyl sulfate (SDS) at 50° C. to 65° C., wherein the nucleic acid sequence consists of SEQ ID NO:1; or
(b) the complement of the nucleic acid sequence.
13. An isolated DNA comprising:
(a) a nucleic acid sequence that (i) encodes a polypeptide that enhances spreading of a macrophage or a monocyte and (ii) hybridizes to the complement of SEQ ID NO:13 under the following conditions: hybridization in 6× SSC at 30° C., followed by one or more washes in 0.2× SSC and 0.1% sodium dodecyl sulfate (SDS) at 50° C. to 65° C., wherein the nucleic acid sequence consists of SEQ ID NO:11; or
(b) the complement of the nucleic acid sequence.
14. An isolated DNA comprising:
(a) a nucleic acid sequence that (i) encodes a polypeptide that enhances spreading of a macrophage or a monocyte and (ii) hybridizes to the complement of SEQ ID NO:13 under the following conditions: hybridization in 6× SSC at 30° C., followed by one or more washes in 0.2× SSC and 0.1% sodium dodecyl sulfate (SDS) at 50° C. to 65° C., wherein the nucleic acid sequence consists of SEQ ID NQ: 9; or
(b) the complement of the nucleic acid sequence.
15. The isolated nucleic acid of claim 3 , wherein the heterologous polypeptide comprises a signal peptide, a reporter polypeptide, or an immunoglobulin constant region.
16. An isolated DNA consisting of a sequence encoding an antigenic fragment of SEQ ID NO:12, wherein the antigenic fragment is at least five amino acids long.
17. An isolated DNA comprising a nucleic acid sequence that encodes a polypeptide consisting of the following segments in contiguous order, starting from the N-terminus of the amino acid sequence:
(a) amino acids 1-30 of SEQ ID NO:12;
(b) amino acids 31-104 of SEQ ID NO:12;
(c) amino acids 105-1267 of SEQ ID NO:12; and
(d) amino acids 1268-1429 of SEQ ID NO:12 or amino acids 1194-1999 of SO ID NO:2,
wherein the polypeptide enhances spreading of a macrophage or a monocyte.
18. The DNA of claim 17 , wherein the polypeptide comprises amino acids 1194-1999 of SEQ ID NO:2.
19. The DNA of claim 17 , wherein the polypeptide comprises amino acids 1268-1429 of SEQ ID NO:12.
20. A vector comprising the isolated DNA of claim 16 .
21. The vector of claim 20 , wherein the nucleic acid sequence is operably linked to a regulatory element that allows expression of the nucleic acid sequence in a cell.
22. An isolated cell comprising the vector of claim 21 .
23. A method of producing a polypeptide, the method comprising culturing the cell of claim 22 and purifying the polypeptide from the cell.
24. A vector comprising the isolated DNA of claim 17 .
25. The vector of claim 24 , wherein the nucleic acid sequence is operably linked to a regulatory element that allows expression of the nucleic acid sequence in a cell.
26. An isolated cell comprising the vector of claim 25 .
27. A method of producing a polypeptide, the method comprising culturing the cell of claim 26 and purifying the polypeptide from the cell.
28. The nucleic acid of claim 3 , wherein a nucleotide sequence encoding the antigenic fragment is at least 50 NUCLEOTIDES long.
29. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 100 nucleotides long.
30. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 300 nucleotides long.
31. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 800 nucleotides long.
32. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 1,500 nucleotides long.
33. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 3,000 nucleotides long.
34. The nucleic acid of claim 28 , wherein the nucleotide sequence is at least 4,000 nucleotides long.
35. The DNA of claim 16 , wherein the a nucleotide sequence encoding the antigenic fragment is at least 50 nucleotides long.
36. The DNA of claim 35 , wherein the nucleotide sequence is at least 100 nucleotides long.
37. The DNA of claim 35 , wherein the nucleotide sequence is at least 300 nucleotides long.
38. The DNA of claim 35 , wherein the nucleotide sequence is at least 800 nucleotides long.
39. The DNA of claim 35 , wherein the nucleotide sequence is at least 1,500 nucleotides long.
40. The DNA of claim 35 , wherein the nucleotide sequence is at least 3,000 nucleotides long.
41. The DNA of claim 35 , wherein the nucleotide sequence is at least 4,000 nucleotides long.
42. An isolated DNA comprising a nucleic acid sequence that encodes a polypeptide consisting of the following segments in contiguous order, starting from the N-terminus of the amino acid sequence:
(a) amino acids 1-30 of SEQ ID NO:12;
(b) amino acids 105-1267 of SEQ ID NO:12; and
(c) amino acids 1268-1429 of SEQ ID NO:12, or amino acids 1194-1999 of SEO ID NO: 2,
wherein the polypeptide enhances spreading of a macrophage or a monocyte.
43. A vector comprising the isolated DNA of claim 42 .
44. Thc vector of claim 43 , wherein the nucleic acid sequence is operably linked to a regulatory element that allows expression of the nucleic acid sequence in a cell.
45. An isolated cell comprising the vector of claim 44 .
46. A method of producing a polypeptide, the method comprising culturing the cell of claim 45 and purifying the polypeptide from the cell.