Inhibition of viral gene activities
A method of inhibiting viral gene activities (and cellular gene activities) is disclosed. In one embodiment, the method comprises the step of delivering an effective amount of an inhibitor of a viral looping/linking factor to an infected patient.
1. A method of screening candidate molecules for the ability to disrupt viral looping/linking factors comprising:
(a) adding a candidate molecule to a mammalian cell culture;
(b) providing a control mammalian cell culture without the candidate molecule, wherein the cell cultures of both (a) and (b) comprise viral looping/linking factors, wherein the factors comprise DNA-binding proteins that can self-associate, and nucleic acid molecules comprising at least two binding sites for the factors, wherein the sites are linked by a looping/linking factor;
(c) allowing said candidate molecule to interact with the viral looping/linking factor present in the mammalian cell culture of step (a); and
(d) directly analyzing the factor for inhibition by the candidate molecule and comparing the result to the results using the control culture, wherein the candidate molecule inhibits protein:protein self-associate between factors as demonstrated by the factor being unable to mediate linking in the presence of the candidate molecule.
2. The method of claim 1 wherein the looping/linking factor is EBNA1.
3. The method of claim 1 wherein the factor binding sites are on a plasmid.
4. A method of screening candidate molecules for the ability to disrupt viral looping/linking factors comprising:
(a) adding a candidate molecule to a mammalian cell culture;
(b) providing a control mammalian cell culture without the candidate molecule, wherein the cell cultures of both (a) and (b) comprise viral looping/linking factors, wherein the factors comprise DNA-binding proteins that can self-associate, and nucleic acid molecules comprising at least two binding sites for the factors, wherein the sites are linked by a looping/linking factor;
(c) allowing said candidate molecule to interact with the viral looping/linking factor present in the mammalian cell culture of step (a); and
(d) analyzing the factor for inhibition by the candidate molecule and comparing the result to the results using the control culture, wherein the candidate molecule inhibits protein:protein self-associate between factors as demonstrated by the factor being unable to mediate linking in the presence of the candidate molecule, wherein the analysis of step (d) is a gel shift assay.
5. A method of screening candidate molecules for the ability to disrupt viral looping/linking factors comprising:
(a) adding a candidate molecule to a mammalian cell culture;
(b) providing a control mammalian cell culture without the candidate molecule, wherein the cell cultures of both (a) and (b) comprise viral looping/linking factors, wherein the factors comprise DNA-binding proteins that can self-associate, and nucleic acid molecules comprising at least two binding sites for the factors, wherein the sites are linked by a looping/linking factor;
(c) allowing said candidate molecule to interact with the viral looping/linking factor present in the mammalian cell culture of step (a); and
(d) analyzing the factor for inhibition by the candidate molecule and comparing the result to the results using the control culture, wherein the candidate molecule inhibits protein:protein self-associate between factors as demonstrated by the factor being unable to mediate linking in the presence of the candidate molecule, wherein the analysis of step (d) is a promoter activation assay.