Small protein that interacts with a ribonucleotide reductase subunit and uses thereof
View Patent ↗The present invention provides for an isolated Sml1 protein or a homologue thereof. The present invention also provides for a screening assay for identifying compounds that are capable of reducing the division rate of a cell by altering an interaction between a ribonucleotide reductase and a Sml1 protein in the cell, which comprises: (a) contacting the cell with a compound, (b) comparing the division rate of the cell in step (a) with the division rate of the cell in the absence of the compound so as to determine whether the compound alters the interaction between the ribonucleotide reductase and the Sml1 protein of the cell, thereby reducing the cell division rate of the cell.
1. A screening assay for identifying a compound that is capable of reducing the division rate of a cell which comprises:
(a) contacting the cell with a compound, wherein the compound consists of a fragment of the Sml1 protein or a peptidomimetic of the Sml1 protein, determined to mimic the binding of Sml1 protein to the large subunit of ribonucleotide reductase (Rnr1), which Sml1 protein comprises amino acids having the amino acid sequence set forth in SEQ ID NO: 2, and
(b) comparing the division rate of the cell in step (a) with the division rate of the cell in the absence of the compound so as to determine whether the compound reduces the division rate of the cell, thereby identifying a compound capable of reducing the division rate of the cell.
2. The screening assay of claim 1 , wherein the fragment is from about 20 amino acids to about 90 amino acids in length.
3. The screening assay of claim 1 , wherein the cell is a yeast cell, a mammalian cell, a plant cell, an insect cell or a microbe.
4. The screening assay of claim 3 , wherein the mammalian cell is a human cell, a hamster cell, a mouse cell, a rat cell or a monkey cell.