Method of determining Dihydropyrimidine dehydrogenase gene expression
The present invention relates to prognostic methods which are useful in medicine, particularly cancer chemotherapy. The object of the invention to provide a method for assessing Dihydropyrimidine dehydrogenase (DPD) expression levels in tissues and prognosticate the probable resistance of a patient's tumor to treatment with 5-FU based therapies by examination of the amount of DPD mRNA in a patient's tumor cells and comparing it to a predetermined threshold expression level. More specifically, the invention provides to oligonucleotide primer pairs DPD3A and DPD3B and methods comprising their use for detecting levels of Dihydropyrimidine dehydrogenase (DPD) mRNA.
1. A method of determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a tissue sample comprising:
(a) obtaining a tumor sample from a patient;
(b) fixing at least a portion of said tumor sample in paraffin to achieve a fixed and paraffin embedded (FPE) tumor tissue sample;
(c) isolating mRNA from said (FPE) tumor tissue sample;
(d) determining the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA isolated from said FPE tumor tissue sample by amplifying the mRNA using an oligonucleotide primer SEQ ID NO: 1 and oligonucleotide primer SEQ ID NO: 2; and
(e) comparing the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA from step(d) to an amount of mRNA of an internal control gene.
2. The method of claim 1 , wherein the RNA is isolated in the presence of an effective amount of chaotropic agent.
3. The method of claim 1 , wherein the tumor sample comprises non-tumor tissue and tumor tissue.
4. A method of determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a tissue sample comprising;
(a) obtaining a tumor sample from a patient;
(b) fixing at least a portion of said tumor sample in paraffin to achieve a fixed and paraffin embedded (FPE) tumor tissue sample;
(c) isolating mRNA from said FPE tumor tissue sample;
(d) determining the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA isolated from said FPE tumor tissue sample by amplifying the mRNA using an oligonucleotide primer SEQ ID NO: 7 and oligonucleotide primer SEQ ID NO: 8; and
(e) comparing the amount of the mRNA from step(d) to an amount of mRNA of an internal control.
5. The method of claim 4 , wherein the mRNA isolated from said FPE tumor tissue is isolated in the presence of an effective amount of chaotropic agent.
6. The method of claim 4 , wherein a FPE tumor tissue sample comprises non-tumor tissue and tumor tissue.
7. A method of determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a tissue sample comprising:
(a) obtaining a tumor sample from a patient, wherein said tumor sample is fixed and paraffin embedded (FPE);
(b) isolating mRNA from said FPE tumor sample, wherein said FPE tumor sample is heated to a temperature in the range of 50 to 100° C.;
c) determining the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA by amplifying the mRNA using an oligonucleotide primer SEQ ID NO: 1, and SEQ ID NO: 2; and
(d) comparing the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA from step (c) to an amount of mRNA of an internal control gene.
8. A method of determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a tissue sample comprising:
(a) obtaining a tumor sample from a patient, wherein said tumor sample is fixed and paraffin embedded (FPE);
(b) isolating mRNA from said FPE tumor sample, wherein said FPE tumor sample is heated to a temperature in the range of 50 to 100° C.;
(c) determining the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA by amplifying the mRNA using an oligonucleotide primer SEQ ID NO: 7, and SEQ ID NO: 8; and
(d) comparing the amount of Dihydropyrimidine dehydrogenase (DPD) mRNA from step (c) to an amount of mRNA of an internal control gene.
9. The method of claim 7 or 8 , wherein said internal control gene is b-actin.
10. The method of claim 7 or 8 , wherein the mRNA is isolated in the presence of an effective amount of chaotropic agent.
11. A method for determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a fixed and paraffin embedded (FPE) tissue sample comprising:
(a) deparaffinizing the FPE tissue sample to obtain a deparaffinized sample;
(b) isolating mRNA from the deparaffinized sample in the presence of an effective amount of a chaotropic agent by first heating the tissue sample in a solution comprising an effective concentration of a chaotropic compound to a temperature in the range of 75° to 100° C. for a time period of 5 to 120 minutes and recovering said mRNA from said chaotropic solution;
(c) subjecting the mRNA to amplification using a pair of oligonucleotide primers capable of amplifying a region of the DPD gene, to obtain an amplified sample; wherein the pair of primers is primer pair SEQ ID NO: 1 and 2 or primer pair SEQ ID NO: 7 and 8; and
(d) determining the quantity of DPD mRNA relative to the quantity of an internal control gene's mRNA.
12. The method of claim 11 wherein, the internal control gene is β-actin.
13. A method for determining the relative level of Dihydropyrimidine dehydrogenase (DPD) gene expression in a fixed and paraffin embedded tissue sample comprising:
(a) deparaffinizing the tissue sample to obtain a deparaffinized sample;
(b) isolating mRNA from the deparaffinized sample by first heating the deparaffinized tissue sample in a solution comprising an effective concentration of a chaotropic agent to a temperature in the range of 50° C. to 100° C. and recovering said mRNA from said solution; and
(c) determining the quantity of DPD mRNA relative to the quantity of an internal control gene's mRNA.
14. A method for determining a platinum-based chemotherapeutic regimen for treating a tumor in a patient comprising:
(a) obtaining a tissue sample of the tumor and fixing the sample to obtain a fixed tumor sample;
(b) isolating mRNA from the fixed tumor sample, wherein the fixed tumor sample is heated in the presence of an effective amount of a chaotropic agent and wherein the heating occurs at a temperature from 50° to 100° C.;
(c) subjecting the mRNA to amplification using a pair of oligonucleotide primers capable of amplifying a region of the Dihydropyrimidine dehydrogenase (DPD) gene to obtain an amplified sample; wherein the pair of primers is primer pair SEQ ID NO:1 and 2 or primer pair SEQ ID NO: 7 and 8;
(d) determining the amount of amplified DPD mRNA in the amplified sample;
(e) comparing the amount of DPD mRNA from step (d) to an amount of mRNA of an internal control gene; and
(f) determining a platinum-based chemotherapeutic regimen based on the amount of DPD mRNA in the amplified sample and a predetermined threshold level for DPD gene expression.
15. The method of claim 14 wherein the fixed tumor sample is heated in the presence of an effective amount of a chaotropic agent and wherein the heating occurs at a temperature from 75° C. to 100° C. for a period of 5 to 120 minutes.