Self-contained devices for detecting biological contaminants
The present invention relates generally to self-contained devices and methods for detecting biological contaminants and, more particularly, to self-contained devices and methods for detecting biological contaminants in relevant settings including, for example, food processing plants, hospitals, medical offices, veterinary offices, and restaurants by using a device or methodology that includes a sampler component and dye component that binds to the biological material in a detectable fashion.
1. A self-contained sampling/testing device comprising:
a. a sampler for collecting target material from a sample; and
b. a signal generator comprising a dye which binds to said target material to signal the presence of said target material,
wherein said sampler contains an absorbent an at the surface of which is positioned a membrane to which the dye is attached either covalently or non covalently.
2. A self-contained sampling/testing device comprising:
a. a sampler for collecting target material from a sample; and
b. a signal generator comprising a dye which binds to said target material to signal the presence of said target material,
wherein said sampler contains an absorbent pad which has the dye attached directly to its surface either covalently or non covalently.
3. The device of claim 1 or 2 , wherein said dye is a protein binding dye.
4. The device of claim 1 or 2 , wherein said target material is protein.
5. The device of claim 1 or 2 , further comprising a separate reagent housing containing an absorbent material and a wetting/neutralizing solution.
6. The device of claim 1 or 2 , wherein said absorbent pad is pre moistened with wetting/neutralizing solution.
7. The device of claim 6 , wherein said wetting/neutralizing solution comprises a neutralizing agent to neutralize any compounds in the sample that might interfere with the binding of said dye to said target material.
8. The device of claim 7 , wherein said neutralizing agent is selected from the group consisting of sodium thiosulfate, MgCl 2 , sodium dodecyl sulfate, tergitol, polyethylene glycol P-1,1,3,3-tetramethylbutylphenyl ether, and polysorbate 20.
9. The device of claim 1 or 2 , wherein said dye is a frequency shift dye of the protein error family.
10. The device of claim 9 , wherein said dye is bromophenol blue.
11. A self-contained sampling/testing device comprising:
(a) a sampler for collecting biological target material from a sample; and
(b) a signal generator comprising a frequency shift dye of the protein error family, wherein said dye binds to said biological target material and produces a color change upon binding to said target material, unbound frequency shift dye need not be separated from biological target bound frequency shift dye in order to detect the presence of target material, and
wherein said sampler contains an absorbent pad at the surface of which is positioned a membrane to which the dye is attached either covalently or non covalently.
12. The device of claim 11 , further comprising a separate reagent housing containing a wetting agent.
13. The device of claim 11 , wherein said dye is bromophenol blue.
14. The device of claim 11 , further comprising a separate reagent housing containing a wetting solution, said solution comprising a neutralizing agent selected from the group consisting of sodium thiosulfate, MgCl 2 , sodium dodecyl sulfate, tergitol, polyethylene glycol P-1,1,3,3-tetramethylbutylphenyl ether, and polysorbate 20.