Monoclonal antibody
The present invention relates to a monoclonal antibody suitable for monitoring the activity of systems involving protein C inhibitor, a method for preparation of the monoclonal antibody, a method for monitoring the activity of systems involving protein C inhibitor and a method for diagnosis of e.g. venous thrombosis, wherein the monoclonal antibody is utilized. The monoclonal antibody is suitable for monitoring the activity of systems involving protein C inhibitor, and it has specific affinity for both i) a complex between a serine proteinase and an inhibitor thereof, and ii) a cleaved and uncomplexed form of the inhibitor, but has substantially no specific affinity for the inhibitor in its uncleaved and uncomplexed form; or a derivative thereof having the same biological activity.
1. A monoclonal antibody suitable for monitoring the activity of systems involving a serpin protein C inhibitor, said monoclonal antibody having specific affinity for both
(i) a complex between a serine proteinase and a serine proteinase inhibitor, and
(ii) a cleaved an uncomplexed form of said serine proteinase inhibitor,
while having no specific affinity for said inhibitor in its uncleaved and active form of said serine proteinase inhibitor, wherein the serine protease inhibitor is a serpin.
2. A monoclonal antibody according to claim 1 , wherein said monoclonal antibody is obtainable by immunisation of an animal with a mixture of
(i) a complex between a serine proteinase and a serine proteinase inhibitor, and
(ii) a cleaved and uncomplexed form of said serine proteinase inhibitor,
followed by screening for and isolation of said monoclonal antibody, wherein the serine proteinase inhibitor is a serpin.
3. A monoclonal antibody according to claim 2 , wherein said animal is a mouse, preferably a Balb/c mouse.
4. A monoclonal antibody according to claim 1 , wherein said serine proteinase is selected from the group consisting of activated protein C (APC), thrombin, coagulation factor X a , trypsin, chymotrypsin, urokinase plasminogen activator (uPA), tissue type plasminogen activator (tPA), plasma kallikrein, factor Xl a , human glandular kallikrein 1 (HGK1) and prostatic specific antigen (PSA).
5. A monoclonal antibody according to claim 1 , wherein said inhibitor is protein C inhibitor (PCI) or α 1 -antitrypsin.
6. A method for preparation of a monoclonal antibody as defined in claim 1 , wherein an animal is immunised with a mixture of
i) a complex between a serine proteinase and a serine proteinase inhibitor, and
ii) a cleaved form of said serine proteinase inhibitor, followed by screening for and isolation of said monoclonal antibody, wherein the serine proteinase inhibitor is a serpin.
7. A method for preparation of a monoclonal antibody according to claim 6 , wherein said animal is a mouse, preferably a Balb/c mouse.
8. A method for monitoring the activity of systems involving protein C inhibitor, wherein a monoclonal antibody as defined in claim 1 is used in an immunoassay.
9. A method according to claim 8 , wherein said immunoassay comprises a sandwich-type immunoassay.
10. A method according to claim 9 , wherein said sandwich-type immunoassay is a technique comprising a tracer agent and said monoclonal antibody bound to a surface.
11. A method according to claim 10 , wherein said tracer agent comprises an antibody having specific affinity for said serine proteinase or an epitope shared by said serine proteinase and said inhibitor.
12. A method according to claim 11 , wherein said tracer agent is conjugated to a enzyme and/or labelled with a tracing substance.
13. A method according to claim 12 , wherein said enzyme is an alkaline phosphatase, horse radish peroxidase or a β-galactosidase.
14. A method according to claim 13 , wherein said tracing substance is 125 I, 131 I, Eu 3+ or Sm 3+ .
15. A kit for qualitative or quantitative determination of the activity of systems involving protein C inhibitor comprising a monoclonal antibody according to claim 1 .
16. A monoclonal antibody according to claim 2 , wherein said serine proteinase is selected from the group consisting of activated protein C (APC), thrombin, coagulation factor X a , trypsin, chymotrypsin, urokinase plasminogen activator (uPA), tissue type plasminogen activator (tPA), plasma kallikrein, factor Xl a , human glandular kallikrein 1 (HGK1) and prostatic specific antigen (PSA).
17. A monoclonal antibody according to claim 2 , wherein said inhibitor is protein C inhibitor (PCI) or α 1 -antitrypsin.
18. A method for preparation of a monoclonal antibody as defined in claim 2 , wherein an animal is immunised with a mixture of
i) a complex between a serine proteinase and a serine proteinase inhibitor, and
ii) a cleaved form of said serine proteinase inhibitor, followed by screening for and isolation of said monoclonal antibody, wherein the serine proteinase inhibitor is a serpin.
19. A method for monitoring the activity of systems involving protein C inhibitor, wherein a monoclonal antibody as defined in claim 2 is used in an immunoassay.
20. A kit for qualitative or quantitative determination of the activity of systems involving protein C inhibitor comprising a monoclonal antibody according to claim 2 .