Method for detecting a designated genetic sequence in murine genomic DNA
The present invention provides a method and apparatus for to conduct transgenic and targeted mutagenesis screening of genomic DNA. This invention also provides a system for screening DNA for a designated genetic sequence. The system includes a computer having a processor, memory and web browser, wherein the computer receives instructions concerning the designated genetic sequence and other screening parameter selection from a remote user via a form of electronic communication, and an automatic screening device that analyzes samples of genomic DNA for the designated sequence.
1. In a method for detecting a designated genetic sequence in a sample of murine genomic DNA, wherein said sample of murine genomic DNA obtained from a lysed tissue sample, in a solid phase hybridization assay including a probe specific for a portion of said murine genomic DNA, the improvement comprising:
separating said murine genomic DNA from said lysed tissue sample using magnetic particles and immobilizing said murine genomic DNA on to a solid phase.
2. The method of claim 1 wherein said designated genetic sequence is a transgenic insert.
3. The method of claim 1 wherein said designated genetic sequence is a selectable marker.
4. The method of claim 3 wherein said selectable marker is selected from a group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
5. The method of claim 1 further comprising the step of adding at least one labeled probe specific for a reference genetic sequence.
6. The method of claim 5 wherein said reference genetic sequence is selected from the group consisting of SEQ ID NO:6, SEQ: ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and SEQ:ID NO:16.
7. The method of claim 1 further comprising the step of adding a morphological control with said genomic DNA prior to depositing said murine genomic DNA on said solid phase.
8. The method of claim 7 wherein said morphological control is lambda DNA.
9. The method of claim 1 wherein said solid phase is an optically flat substrate.
10. The method of claim 9 wherein said optically flat substrate is glass.
11. The method of claim 9 wherein said optically flat substrate is plastic.
12. The method of claim 9 wherein said optically flat substrate is a membrane.
13. The method of claim 9 wherein said optically flat substrate is functionalized with a chemical moiety that bonds with said murine genomic DNA.
14. The method of claim 9 wherein said murine genomic DNA is immobilized on said optically flat substrate by ultraviolet cross-linking.
15. The method of claim 9 wherein said murine genomic DNA is immobilized on said optically flat substrate by heating.
16. The method of claim 9 wherein said optically flat substrate has a sufficient number of aldehyde groups to immobilize said murine genomic DNA.
17. The method of claim 9 wherein said optically flat substrate has a sufficient number of amino group to immobilize said murine genomic DNA.
18. The method of claim 9 wherein said optically flat substrate has a sufficient number of polylysines to immobilize said murine genomic DNA.