Methods for increasing plant cell proliferation by functionally inhibiting a plant cyclin inhibitor gene
The present invention provides methods for modulating the growth and/or yield of plants. In particular the methods comprise the use of agents which functionally inhibit the expression of plant D-like cyclin inhibitors including isolated polynucleotide sequences which interact with DNA or RNA encoding proteins capable of binding plant D-like cyclins. Further, the present invention provides recombinant polynucleotide sequences, vectors and host cells which encode proteins capable of binding to and inactivating the activity of plant D-like cyclin/cyclin dependent kinase complexes preventing plant cells from exiting the cell cycle. Methods for determining and agents which are inhibitors of the BRO cyclin dependent kinase inhibitor proteins which are capable of modulating plant cell cycle progression are also provided. Methods for the production of transgenic plant cells and plants with increased growth rates and yields when compared to wild-type plants are also provided.
1. An isolated nucleotide sequence which encodes the plant D-like cyclin inhibitor protein designated BRO4 as depicted in SEQ ID NO: 8 initiating at amino acid residue position 13 and terminating at amino acid residue position 208.
2. The isolated nucleotide sequence of claim 1 , wherein the nucleotide sequence is that depicted in SEQ ID NO: 7.
3. A vector comprising the nucleotide sequence of claim 1 .
4. The vector according to claim 3 , wherein the vector further comprises a selectable marker.
5. The vector according to claim 3 , wherein the vector further comprises an operably linked promoter that functions in a plant cell.
6. The vector according to claim 5 , wherein the promoter is associated with the gene encoding CaMV 35S, tomato E8, patatin, ubiquitin, mannopine synthase, rice actin I, soybean seed protein glycinin (Gly1), soybean vegetative storage protein, or is the hybrid promoter MAC comprising elements of the promoter associated with the gene encoding CaMV 35S and the 3′ region of the gene encoding mannopine synthase.
7. A host cell comprising the vector of claim 3 .
8. A host cell comprising the vector of claim 4 .
9. A host cell comprising the vector of claim 5 .
10. A host cell comprising the vector of claim 6 .