Tripeptide of FcγRIIA
View Patent ↗The present invention relates, in general, to phagocytosis and phagolysosomal fusion and, in particular, to a tripeptide of FcγRIIA that mediates trafficking of targets phagocytosed via FcγRIIA to the lysosomal compartment.
1. A method of enhancing the ability of a cell to degrade a particle comprising introducing into said cell a nucleic acid sequence encoding an Fc receptor comprising an L-T-L sequence in a cytoplasmic domain thereof, said introduction being effected under conditions such that said nucleic acid sequence is expressed and said enhancement is thereby effected,
wherein said Fc receptor comprises a FcγRIIA cytoplasmic domain modified to comprise at least 1 additional L-T-L peptide.
2. The method according to claim 1 wherein said cell naturally expresses FcγRIIA.
3. The method according to claim 1 wherein said cell does not naturally express FcγRIIA.
4. The method according to claim 1 wherein said cell is an endothelial cell, a fibroblast, a macrophage or an epithelial cell.
5. The method according to claim 1 wherein said particle is a bacterium.
6. The method according to claim 1 wherein said nucleic acid sequence is introduced into said cell in a liposome, a bacterium or a viral vector.