Method for moving charged particles
Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules through the medium. The devices although preferably small in size, preferably generate large numbers (100 or more) of electrical fields to a movement area which preferably contains a liquid buffered or gel medium. Mixtures of charged molecules are pulled through the gel by the force of the electrical fields. The fields are preferably activated simultaneously or sequentially one after another at various speeds to create complex force field distributions or moving field waves along the separation medium. Charged molecules capable of moving quickly through the gel will be moved along by the faster moving field waves and be separated from slower moving molecules. The fields can be activated by computer software and can be used to move molecules away from and toward each other to obtain rapid and complex chemical synthesis, sequencing or reaction protocols.
1. A method for separating different charged particles in a medium employing an electrical field, said method comprising:
providing a device comprising (a) a substrate having an upper surface, (b) a main trench in the substrate extending downward from the upper surface, (c) a plurality of electrodes positioned at intervals along the trench such as to be in electrical contact with a medium when present in the trench, the plurality of electrodes being connected to an electronic computer programmed to sequentially activate electrodes to provide electrical field waves that move along the trench at a speed, and (d) an optical detection device positioned along the trench to detect charged particles in the medium in the trench, the optical detection device being interactive with the electronic computer so that the speed of the electrical field waves can be adjusted based on charged particles detected by the optical detection device;
placing a sample of the charged particles into the medium in the device; and
separating the different charged particles by electrical field waves having speeds determined by the electronic computer in interaction with the optical detection device.
2. The method of claim 1 , wherein said substrate is an organic polymer solid substrate.
3. The method of claim 2 , wherein said organic polymer solid substrate is polymethylmethacrylate, polycarbonate, polyethylene terephthalate, or polystyrene.
4. The device of claim 2 wherein said organic polymer solid substrate has a substantially uncharged surface.
5. The device of claim 1 wherein said main trench has capillary dimensions.
6. The method of claim 1 , wherein said intervals are between 1 and 100 microns in distance.
7. The method of claim 1 , wherein said intervals are regular intervals.