Non-human primate Fc receptors and methods of use
The invention provides isolated non-human primate Fc receptor polypeptides, the nucleic acid molecules encoding the Fc receptor polypeptides, and the processes for production of recombinant forms of the Fc receptor polypeptides, including fusions, variants, and derivatives thereof. The invention also provides methods for evaluating the safety, efficacy and biological properties of Fc region containing molecules using the non-human primate Fc receptor polypeptides.
1. An isolated nucleic acid comprising a polynucleotide sequence that encodes a non-human primate Fc receptor polypeptide with an amino acid sequence of SEQ ID NO: 9 or a fragment corresponding to amino acids 1 to 269 of SEQ ID NO: 65.
2. An isolated nucleic acid of claim 1 , wherein the polynucleotide sequence has a sequence of SEQ ID NO: 1.
3. An isolated nucleic acid encoding an Fc receptor polypeptide prepared according to the method comprising:
a) amplifying a nucleic acid from a nonhuman primate cell with a primer set comprising a forward and a reverse primer, wherein the primer sets are selected from SEQ ID NO:31 and SEQ ID NO:32 or SEQ ID NO:33 and SEQ ID NO:34; and
b) isolated the amplified nucleic acid.
4. An isolated nucleic acid of claim 1 , wherein the polynucleotide encodes an extracellular fragment of the Fc receptor polypeptide with an amino acid sequence corresponding to amino acids 1 to 269 of SEQ ID NO:65.
5. A vector comprising a nucleic acid of claim 1 .
6. A host cell comprising a vector of claim 5 .
7. A host according to claim 6 , wherein the cell is a mammalian cell.
8. A nucleic acid of claim 1 , further comprising a polypeptide nucleotide sequence encoding a heterologous polypeptide operably linked to the nucleotide sequence encoding a Fc receptor polypeptide, wherein the heterologous polypeptide is selected from the group consisting of Gly/His 6 fused to glutathione S-transferase, 6-His tag, thioredoxin tag, hemaglutinin tag, Glylh156 tag, and OmpA signal sequence tag.
9. An isolated nucleic acid comprising a polynucleotide sequence that encodes a non-human primate Fc receptor polypeptide with an amino acid sequence of SEQ ID NO: 65 or a fragment corresponding t 6 o amino acids 1 to 269 of SEQ ID NO: 65.
10. An isolated nucleic acid of claim 9 , wherein the polynucleotide sequence has a sequence of SEQ ID NO: 1.
11. A vector comprising a nucleic acid of claim 9 .
12. A host cell comprising a vector of claim 11 .
13. A host according to claim 12 , wherein the cell is a mammalian cell.
14. A nucleic acid of claim 9 , further comprising a polypeptide nucleotide sequence encoding a heterologous polypeptide operably linked to the nucleotide sequence encoding a Fc receptor polypeptide, wherein the heterologous polypeptide is selected from the group consisting of Gly/His 6 fused to glutathione S-transferase, 6-His tag, thioredoxin tag, hemaglutinin tag, Glyh156 tag, and OmpA signal sequence tag.