Inhibitor of apoptosis proteins and nucleic acids and methods for making and using them
View Patent ↗The invention provides polypeptides comprising inhibitor of apoptosis protein (IAP) family members, such as BmIAP initially derived from Bombyx mori BmN cells, and nucleic acids encoding them, and methods for making and using these compositions, including their use for inhibiting apoptosis.
1. An isolated nucleic acid encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide is capable of inhibiting caspase 9 .
2. The isolated nucleic acid of claim 1 , wherein the polypeptide is capable of inhibiting Bax-induced apoptosis in Spodoptera frugipera or Bombyx mori cells.
3. The isolated nucleic acid of claim 1 , wherein the polypeptide is capable of inhibiting Bax -induced apoptosis in mammalian cells.
4. An isolated nucleic acid encoding a polypeptide comprising SEQ ID NO:2.
5. An isolated nucleic acid comprising SEQ ID NO:1.
6. An isolated expression cassette comprising at least one nucleic acid operably linked to a promoter, wherein the nucleic acid encodes a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide is capable of inhibiting caspase 9 .
7. The isolated expression cassette of claim 6 , wherein the promoter is a constitutive promoter.
8. The isolated expression cassette of claim 6 , wherein the promoter is a developmentally regulated or a tissue specific promoter.
9. The isolated expression cassette of claim 6 , wherein the polypeptide comprises SEQ ID NO:2.
10. An isolated cell transformed with an expression vector comprising a nucleic acid encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide is capable of inhibiting caspase 9 .
11. The isolated cell of claim 10 , wherein the cell is a mammalian cell.
12. The isolated cell of claim 10 , wherein the cell is an insect cell.
13. The isolated cell of claim 12 , wherein the insect cell is a Spodoptera frugipera cell or a Bombyx mori cell.
14. The isolated cell of claim 10 , wherein the cell is a plant cell.
15. The isolated cell of claim 10 , wherein the cell is a yeast cell.
16. The isolated cell of claim 10 , wherein the polypeptide comprises SEQ ID NO:2.
17. An array comprising a nucleic acid encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide is capable of inhibiting caspase 9 .
18. A method of making a polypeptide having at least 95% sequence identity to SEQ ID NO:2 comprising culturing a cell comprising an expression vector comprising a nucleic acid encoding said polypeptide wherein said polypeptide is capable of inhibiting caspase 9 .
19. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises a BIR1 domain having the amino acid sequence of residues 74 to 140 of SEQ ID NO:2, BIR2 domain having the amino acid sequence of residues 182 to 249 of SEQ ID NO:2, and a RING domain having the amino acid sequence of residues 298 to 314 of SEQ ID NO:2.
20. The isolated expression cassette of claim 6 , wherein the nucleic acid comprises SEQ ID NO:1.
21. The isolated expression cassette of claim 6 , wherein the polypeptide comprises a BIR1 domain having the amino acid sequence of residues 74 to 140 of SEQ ID NO:2, a BIR2 domain having the amino acid sequence of residues 182 to 249 of SEQ ID NO:2, and a RING domain having the amino acid sequence of residues 298 to 314 of SEQ ID NO:2.
22. The isolated cell of claim 10 , wherein the nucleic acid comprises SEQ ID NO:1.
23. The isolated cell of claim 10 , wherein the polypeptide comprises a BIR1 domain having the amino acid sequence of residues 74 to 140 of SEQ ID NO:2, a BIR2 domain having the amino acid sequence of residues 182 to 249 of SEQ ID NO:2, and a RING domain having the amino acid sequence of residues 298 to 314 of SEQ ID NO:2.
24. The array of claim 17 , wherein the polypeptide comprises SEQ ID NO:2.
25. The array of claim 24 , wherein the nucleic acid comprises SEQ ID NO:1.
26. The array of claim 17 , wherein the polypeptide comprises a BIR1 domain having the amino acid sequence of residues 74 to 140 of SEQ ID NO:2, a BIR2 domain having the amino acid sequence of residues 182 to 249 of SEQ ID NO:2, and a RING domain having the amino acid sequence of residues 298 to 314 of SEQ ID NO:2.
27. The method of claim 18 , wherein the polypeptide comprises SEQ ID NO:2.
28. The array of claim 27 , wherein the nucleic acid comprises SEQ ID NO:1.
29. The method of claim 18 , wherein the polypeptide comprises a BIR1 domain having the amino acid sequence of residues 74 to 140 of SEQ ID NO:2, a BIR2 domain having the amino acid sequence of residues 182 to 249 of SEQ ID NO:2, and a RING domain having the amino acid sequence of residues 298 to 314 of SEQ ID NO:2.