Nucleic acid encoding G-protein coupled receptor with modified DRY motif
The present invention relates to modified G-protein coupled receptors (GPCRs). The modified GPCRs of the present invention include GPCRs that have been modified to have altered DRY motifs such that the modified GPCRs are constitutively desensitized. As such, the modified GPCRs of the present invention preferably localize to endocytic vesicles or endosomes in an agonist-independent manner. The invention also relates to methods of screening compounds and sample solutions for GPCR activity using the modified GPCRs.
1. A nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.
2. A nucleic acid encoding a polypeptide selected from the group consisting of SEQ ID NOs.: 1–6.
3. A vector comprising a nucleic acid of claim 1 .
4. An isolated host cell comprising the expression vector of claim 3 .
5. A nucleic acid encoding a modified GPCR or biologically active fragment thereof comprising a DRY motif modified to contain an amino acid other than arginine at position 2, wherein the modified GPCR or biologically active fragment thereof is constitutively desensitized in the absence of an agonist, wherein said nucleic acid is at least 80% identical to a nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.
6. A nucleic acid encoding a modified GPCR or biologically active fragment thereof comprising a DRY motif modified to contain an amino acid other than arginine at position 2, wherein the modified GPCR or biologically active fragment thereof is constitutively desensitized in the absence of an agonist, wherein said nucleic acid is at least 90% identical to a nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.
7. A nucleic acid encoding a modified GPCR or biologically active fragment thereof comprising a DRY motif modified to contain an amino acid other than arginine at position 2, wherein the modified GPCR or biologically active fragment thereof is constitutively desensitized in the absence of an agonist, wherein said nucleic acid is at least 95% identical to a nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.
8. A nucleic acid encoding a modified GPCR or biologically active fragment thereof comprising a DRY motif modified to contain an amino acid other than arginine at position 2, wherein the modified GPCR or biologically active fragment thereof is constitutively desensitized in the absence of an agonist, wherein said nucleic acid is at least 98% identical to a nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.
9. A nucleic acid encoding a modified GPCR or biologically active fragment thereof comprising a DRY motif modified to contain an amino acid other than arginine at position 2, wherein the modified GPCR or biologically active fragment thereof is constitutively desensitized in the absence of an agonist, wherein said nucleic acid is at least 99% identical to a nucleic acid selected from the group consisting of SEQ ID NOs: 7–12.