Methods for altering organ mass in plants
View Patent ↗The invention provides methods of altering organ mass, controlling fertility and enhancing asexual reproduction in plants through the modulation of plant growth and cell proliferation. The methods involve producing transgenic plants comprising a recombinant expression cassette containing a modified ANT nucleic acid linked to a plant promoter.
1. A method of increasing cell proliferation in a plant, the method comprising introducing into the plant an expression cassette containing a plant promoter operably linked to an ANT nucleic acid encoding a truncated ANT polypeptide having no more than about 500 amino acids, said truncated ANT polypeptide comprising the amino acid sequence of positions 281–451 of SEQ ID NO:2; and selecting plants with increased size or mass when compared to a wild-type plant.
2. The method of claim 1 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:13.
3. The method of claim 1 , wherein the plant has increased floral organ mass.
4. The method of claim 1 , wherein the plant has increased vegetative organ mass.
5. The method of claim 1 , wherein the step of introducing is carried out using Agrobacterium.
6. The method of claim 1 , wherein the promoter is heterologous to the ANT nucleic acid encoding the truncated ANT polypeptide.
7. The method of claim 6 , wherein the promoter is a constitutive promoter.
8. The method of claim 7 , wherein the promoter is CaMV 35S promoter.
9. The method of claim 6 , wherein the promoter is a tissue specific promoter.
10. The method of claim 1 , wherein the plant is Arabidopsis.
11. A recombinant expression cassette comprising plant promoter operably linked to an ANT nucleic acid encoding a truncated ANT polypeptide having no more than about 500 amino acids, said truncated ANT polypeptide comprising the amino acid sequence of positions 281–451 of SEQ ID NO:2.
12. The recombinant expression cassette of claim 11 , wherein the promoter is heterologous to the ANT nucleic acid encoding the truncated ANT polypeptide.
13. The recombinant expression cassette of claim 12 , wherein the promoter is a constitutive promoter.
14. The recombinant expression cassette of claim 13 , wherein the promoter is CaMV 35S promoter.
15. The recombinant expression cassette of claim 12 , wherein the promoter is a tissue specific promoter.
16. The recombinant expression cassette of claim 11 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:13.
17. The method of claim 1 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:9.
18. The method of claim 1 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:10.
19. The recombinant expression cassette of claim 11 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:9.
20. The recombinant expression cassette of claim 11 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:10.
21. The method of claim 1 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:11.
22. The method of claim 1 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:15.
23. The recombinant expression cassette of claim 11 , wherein the truncated ANT polyprptide comprises a sequence as shown in NO:11.
24. The recombinant expression cassette of claim 11 , wherein the truncated ANT polypeptide comprises a sequence as shown in SEQ ID NO:15.