Expansion of hematopoietic cells using midkine or pleiotrophin
The present invention provides novel use of the MK family that is used alone as an agent for proliferating hematopoietic stem cells and hematopoietic precursor cells. The invention also provides an agent for remarkably enhancing the above-described effect for promoting the proliferation of hematopoietic stem cells and hematopoietic precursor cells, comprising the MK family in combination with known hematopoietic factors such as IL-3, IL-6, G-CSF, GM-CSF, M-CSF, SCF, and EPO.
1. A method for promoting ex vivo expansion of hematopoietic stem cells or hematopoietic progenitor cells, said method comprising culturing hematopoietic stem cells or hematopoietic progenitor cells with a composition comprising: (a) purified midkine (MK) protein or purified pleiotrophin (PTN) protein; and (b) one or more other purified hematopoietic growth factors selected from the group consisting of interleukin-3 (IL-3), interleukin-6 (IL-6), granulocyte colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), and erythropoietin (EPO).
2. A method for promoting ex vivo expansion of hematopoietic stem cells or hematopoietic progenitor cells, said method comprising culturing hematopoietic stem cells or hematopoietic progenitor cells with a composition comprising: (a) purified midkine (MK) protein; and (b) one or more other purified hematopoietic growth factors selected from the group consisting of interleukin-3 (IL-3), interleukin-6 (IL-6), granulocyte colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), and erythropoietin (EPO).
3. The method of claim 1 , wherein the hematopoietic stem cells or hematopoietic progenitor cells are obtained from bone narrow, peripheral blood, or umbilical cord blood.
4. The method of claim 1 , wherein the hematopoietic stem cells are colony-forming units-mix (CFU-mix).
5. The method of claim 1 , wherein the other purified hematopoietic growth factors are IL-3, IL-6, SCF, and EPO.
6. The method of claim 2 , wherein the hematopoietic stem cells or hematopoietic progenitor cells are obtained from bone marrow, peripheral blood, or umbilical cord blood.
7. The method of claim 2 , wherein the hematopoietic stem cells are colony-farming units-mix (CFU-mix).
8. The method of claim 2 , wherein the other purified hematopoietic growth factors are IL-3, IL-6, SCF, and EPO.