Induction of tumor immunity by variants of folate binding protein
The present invention is directed to variants of antigens comprising folate binding protein epitopes as a composition associated with providing immunity against a tumor in an individual. The variant is effective in inducing cytotoxic T-lymphocytes but preferably not to the extent that they become sensitive to silencing by elimination, such as by apoptosis, or by energy, as in unresponsiveness.
1. A peptide comprising the amino acid sequence of a variant of a peptide fragment of the polypeptide of SEQ ID NO:10, wherein said peptide is of at least 10 amino acids or up to about 30 amino acids, comprises SEQ ID NO:268 but for substitution therein of the amino acids at positions 1 and 7 thereof by phenylalanine and threonine, respectively, and further comprises from 1 to 21 additional contiguous amino acids of the amino acid sequences of SEQ ID NO:10 that flank SEQ ID NO:268 as contained therein.
2. A peptide comprising the amino acid sequence of the peptide of claim 1 and the amino acid sequence of at least one additional peptide, wherein said additional peptide is of at least 9 amino acids or up to about 30 amino acids and comprises an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:268 but for substitution therein of the amino acid at position 5 thereof by phenylalanine;
(b) the amino acid sequence of SEQ ID NO:268 but for substitution therein of the amino acids at positions 6 and 7 thereof by alanine and threonine, respectively;
(c) the amino acid sequence of SEQ ID NO:268 but for substitution therein of the amino acids at positions 5 and 7 thereof by phenylalanine and threonine, respectively;
(d) the amino acid sequence of SEQ ID NO:268 but for substitution therein of the amino acids at positions 1, 5, and 7 thereof by phenylalanine, phenylalanine, and threonine, respectively; and
(e) the amino acid sequence of SEQ ID NO:268 but for substitution therein of the amino acids at positions 1 and 7 thereof by glycine and threonine, respectively.