Single and multi-aperture, translationally-coupled confocal microscope
Disclosed are microscopes, preferably confocal microscopes, that include aperture plates having one or more arrays of apertures defined therein. The aperture plate is disposed in a position within an incoming light beam such that the incoming light beam is spatially filtered as it passes through the array of apertures. In certain embodiments of the invention, the aperture plate is fixed and immovable, in others, the aperture plate is translatable within a plane of confocality.
1. A microscope comprising:
a light source for emitting an incoming light beam with which a sample to be observed is irradiated, thereby generating a return light beam from the sample;
a fixed and immovable aperture plate having an array of apertures defined therein,
wherein the aperture plate is disposed in a position within the incoming light beam such that the incoming light beam is spatially filtered as it passes through the array of apertures; and
a first means for scanning the spatially-filtered incoming light beam from the aperture plate across the sample and for de-scanning the return light beam from the sample so that the return light beam impinges on the aperture plate; and
further comprising photodetection means disposed within the return light beam at a position capable of detecting the return light beam after it has passed through the array of apertures; and
further comprising second means for scanning the return light beam, the second means disposed within the return light beam at a point after the return light beam has passed through the array of apertures, but prior to the return light beam reaching the photodetection means.
2. The microscope of claim 1 , wherein the second means for scanning is selected from the group consisting of a galvanometer-controlled mirror, an acousto-optical deflector, a polygonal scanner, a diffraction grating, and a microelectromechanical system.
3. A microscope comprising:
a light source for emitting an incoming light beam with which a sample to be observed is irradiated, thereby generating a return light beam from the sample;
a fixed and immovable aperture plate having an array of apertures defined therein,
wherein the aperture plate is disposed in a position within the incoming light beam such that the incoming light beam is spatially filtered as it passes through the array of apertures;
a first means for scanning the spatially-filtered incoming light beam from the aperture plate across the sample and for de-scanning the return light beam from the sample so that the return light beam impinges on the aperture plate;
wherein the fixed aperture plate has at least two distinct arrays of apertures defined therein; wherein a first of the arrays of apertures is disposed in a fixed position within the incoming light beam such that the incoming light beam is spatially filtered as it passes through the first array of apertures; and wherein a second of the arrays of apertures is disposed in a fixed position within the return light beam such that the return light beam is spatially filtered as it passes through the second array of apertures;
photodetection means disposed within the return light beam at a position capable of detecting the return light beam after it has passed through the second array of apertures; and
second means for scanning the return light beam, the second means disposed within the return light beam at a point after the return light beam has passed through the second array of apertures, but prior to the return light beam reaching the photodetection means.
4. The microscope of claim 3 , wherein the first means for scanning and the second means for scanning are independently selected from the group consisting of a galvanometer-controlled mirror, an acousto-optical deflector, a polygonal scanner, a diffraction grating, and a microelectromechanical system.
5. The microscope of claim 3 , wherein the photodetection means is disposed within the return light beam at a position directly behind the second array of apertures.