Human mullerian duct-derived epithelial cells and methods of isolation and uses thereof
This invention discloses a substantially pure population of human Müllerian duct-derived epithelial cells and methods of isolating and culturing the Müllerian duct-derived epithelial cells. By carefully manipulating the microenvironment in which the Müllerian duct-derived epithelial cells are grown, multiple passages are attainable wherein the Müllerian duct-derived epithelial cells are capable of becoming uterine, cervical, vaginal, and oviductal cells. In addition, several uses of human Müllerian duct-derived epithelial cells and cells differentiating therefrom are disclosed herein.
1. A method of providing a source of an immunogen to a heterologous recipient, comprising: administering to said recipient a substantially pure population of human Müllerian duct-derived epithelial cells in an amount effective to induce an immune response in said recipient; wherein said Müllerian duct-derived epithelial cells have a pluripotent capacity to differentiate into uterine, oviductal, cervical, and vaginal cells.
2. The method of claim 1 , further comprising maintaining the Müllerian duct-derived epithelial cells in serum-free media before administering the Müllerian duct-derived epithelial cells to said recipient.
3. The method of claim 1 , wherein cell surfaces of said Müllerian duct-derived epithelial cells are substantially free of serum biomolecules.
4. The method of claim 1 , further comprising identifying the expression of at least one cell surface marker in said Müllerian duct-derived epithelial cells before administering said Müllerian duct-derived epithelial cells to said recipient, wherein said at least one cell surface marker is selected from the group consisting of cytokeratin 1, cytokeratin 5, cytokeratin 6, cytokeratin 7, cytokeratin 8, cytokeratin 10, cytokeratin 11, cytokeratin 13, cytokeratin 15, cytokeratin 16, cytokeratin 18, cytokeratin 19, and vimentin.