Screen for selective inhibitors or activators of Smad protein function
A method of screening for selective inhibitors or activators Smad protein function is disclosed. In one embodiment, the invention comprises the steps of (a) obtaining a phosphorylated Smad protein or protein complex, (b) allowing the phosphorylated Smad protein or complex to interact with a target protein or peptide in the presence of a test compound, and (c) analyzing the binding of the phosphorylated Smad complex or protein and the target protein or peptide, wherein a perturbation of binding indicates that the test compound is an inhibitor or activator of Smad-target protein interaction.
1. A method of screening for selective inhibitors or activators of Smad-target protein binding comprising the steps of:
(a) obtaining a phosphorylated Smad protein or phosphorylated Smad protein complex,
(b) allowing the phosphorylated Smad protein or protein complex to interact with a target protein or peptide in the presence of a test compound, and
(c) analyzing the binding of the phosphorylated Smad protein or protein complex and the target protein or peptide, wherein a perturbation of binding indicates that the test compound is an inhibitor or activator of Smad-target protein interaction and wherein at least 90 samples are analyzed simultaneously.
2. The method of claim 1 wherein the phosphorylated Smad protein comprises a protein selected from the group consisting of Smad2 and Smad3.
3. The method of claim 1 wherein the phosphorylated Smad complex comprises Smad2 and Smad4.
4. The method of claim 1 wherein the target protein is FAST.
5. A method of screening for selective inhibitors or activators of Smad-target protein binding comprising the steps of:
(a) obtaining a phosphorylated Smad protein or phosphorylated Smad protein complex,
(b) allowing the phosphorylated Smad protein or protein complex to interact with a target protein or peptide in the presence of a test compound, and
(c) analyzing the binding of the phosphorylated Smad protein or protein complex and the target protein or peptide, wherein a perturbation of binding indicates that the test compound is an inhibitor or activator of Smad-target protein interaction and wherein step (c) is via homogeneous time resolved fluorescence.
6. A method of screening for selective inhibitors or activators of Smad-target protein binding comprising the steps of:
(a) obtaining a phosphorylated Smad protein or phosphorylated Smad protein complex,
(b) allowing the phosphorylated Smad protein or protein complex to interact with a target protein or peptide in the presence of a test compound, and
(c) analyzing the binding of the phosphorylated Smad protein or protein complex and the target protein or peptide, wherein a perturbation of binding indicates that the test compound is an inhibitor or activator of Smad-target protein interaction, wherein step (c) is via solid phase ELISA assay.