Method for removing multimers of human serum albumin
View Patent ↗Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.
1. A method for purifying a recombinant human serum albumin monomer from its multimers, comprising:
(1)treating a recombinant human serum albumin solution at pH 9.0 to convert the multimers of recombinant human serum albumin into monomers, and
(2) loading treated recombinant human serum albumin solution onto an anion-exchange column equilibrated with a buffer with a concentration range from 5 to 200 mM containing added salt concentration ranging from 50 to 100 mM an having a pH value from 6.0 to 7.0 and recovering all of the fractions passed through the column without undergoing absorption.
2. The method of claim 1 , wherein the salt concentration in the buffer in (2) ranges from 50 to 75 mM.
3. The method of claim 1 , wherein the purity of the recombinant human serum albumin in the recombinant human serum albumin solution to be treated is not less than about 85%.
4. The method of claim 2 , wherein the purity of the recombinant human serum albumin in the recombinant human serum albumin solution to be treated is not less than about 85%.
5. The method of claim 2 , wherein the salt is an alkali metal chloride.
6. The method of claim 2 , wherein the salt is sodium chloride.
7. The method of claim 2 , wherein the salt is potassium chloride.
8. The method of claim 1 wherein the buffer has a concentration range of 10–100 mM.