Increased cell resistance to toxic organic substances
Recombinant microorganisms and related methods of use to enhance tolerance to toxic substances. In particular, such microorganisms and methods can be used to increase solvent production.
1. An isolated solventogenic bacterium having a recombinant plasmid comprising genetic code material to increase expression of a heat shock protein, said bacterium comprising a recombinant strain of solventogenic Clostridia.
2. The bacterium of claim 1 wherein said genetic material comprises a DNA sequence selected from the group consisting of the groE operon, the dnaK operon and combinations of said operons.
3. The bacterium of claim 1 wherein said genetic material comprises DNA encoding proteins selected from the group consisting of GroESL proteins, the DnaKJ proteins and combinations thereof.
4. The bacterium of claim 1 comprising constructed pSR1.
5. The bacterium of claim 1 comprising genetic code for expression of the GroESL heat shock proteins.
6. The bacterium of claim 5 comprising the pSOS95del shuttle vector and the groESL operon genes.
7. The bacterium of claim 6 wherein said plasmid comprises the pSOS95del shuttle vector and said bacterium is a recombinant strain of C. acetobutylicum.
8. A method of using expression of a heat shock protein to enhance solvent production, said method comprising:
providing a recombinant solventogenic C. acetobutylicum bacterium, said bacterium transformed by a plasmid comprising genetic code to increase expression of a heat shock protein;
providing a fermentation system conditioned for solvent production; and
contacting said recombinant bacterium with said system over a time sufficient for expression of said heat shock protein and enhanced solvent production compared to production by said bacterium absent said transformation.
9. The method of claim 8 wherein said plasmid comprises genetic code for expression of the GroESL heat shock proteins.
10. A method of using expression of a heat shock protein to increase tolerance to a toxic substance, said method comprising:
providing a recombinant microorganism, said microorganism selected from recombinant strains of solventogenic Clostridia, Aspergillus, Pichia , species of the genus Lactobacilli , and species of the genus Bacillus , said microorganism transformed by a plasmid comprising genetic code to increase expression of a heat shock protein;
providing a fermentation system; and
contacting said recombinant microorganism with said system over a time sufficient for expression of said heat shock protein said increased tolerance compared to tolerance of said recombinant microorganism absent said transformation.
11. The method of claim 10 wherein said recombinant strain comprises genetic code for expression of the GroESL heat shock proteins.
12. The method of claim 10 wherein contacting said recombinant microorganism with said system provides for bioremediation of a toxic substance.
13. The method of claim 10 wherein contacting said recombinant microorganism with said system provides for the biocatalysis of a target compound.
14. The method of claim 13 wherein said microorganism comprises a recombinant microorganism selected from the group consisting of recombinant microorganisms of the genus Escherichia , recombinant microorganisms of the genus Saccharomyces , and recombinant microorganisms of the genus Pichia.
15. A method of using expression of a heat shock protein to increase tolerance to solvent production, said method comprising:
providing a recombinant microorganism, said microorganism transformed by a plasmid comprising genetic code to increase expression of a heat shock protein
providing a fermentation system; and
contacting said recombinant microorganism with said system over a time sufficient for expression of said heat shock protein, said increased solvent tolerance compared to tolerance of said recombinant microorganism absent said transformation.
16. The method of claim 15 wherein said microorganism is selected from the group consisting of bacteria, yeast, and filamentous fungi.
17. The method of claim 15 wherein said microorganism comprises a recombinant strain of C. acetobutylicum.
18. The method of claim 15 wherein said microorganism is a recombinant bacterium species selected from the group consisting of species of the genus Escherichia , species of the genus Lactobacilli and species of the genus Bacillus.
19. The method of claim 17 wherein said recombinant strain comprises genetic code for expression of the GroESL heat shock proteins.
20. The method of claim 15 wherein contacting said recombinant microorganism with said system provides for bioremediation of a toxic substance.
21. The method of claim 20 wherein said microorganism comprises a recombinant species selected from the group consisting of species of the genus Aspergillus.
22. The method of claim 15 wherein contacting said recombinant microorganism with said system provides for the biocatalysis of a target compound.
23. The method of claim 22 wherein said microorganism comprises a recombinant microorganism selected from the group consisting of recombinant microorganisms of the genus Escherichia , recombinant microorganisms of the genus Saccharomyces , and recombinant microorganisms of the genus Pichia.