Embryo sac-specific genes
The present invention relates to isolated nucleotide sequences useful for the production of plants with a modified embryo sac, embryo and/or endosperm development, and to transgenic cells and plants transformed with the nucleotide sequences.
1. An isolated nucleotide sequence comprising a sequence selected from the group consisting of: SEQ ID NO: 4, 8, or 14.
2. The nucleotide sequence according to claim 1 , wherein the nucleotide sequence encodes a requlatory protein having the amino acid sequence of SEQ ID NO: 12.
3. The nucleotide sequence of claim 1 comprising the sequence of SEQ ID NO: 14, or the complementary strand thereof.
4. The nucleotide sequence of claim 1 , wherein the sequence is obtained from maize.
5. The nucleotide sequence of claim 1 , wherein the sequence is a DNA, cDNA or RNA molecule.
6. A vector comprising the nucleotide sequence of claim 1 .
7. The vector of claim 6 , which is a bacterial vector or viral vector.
8. The vector of claim 6 , wherein the nucleotide sequence of is operably linked to at least one regulatory element in antisense or sense orientation.
9. The vector of claim 6 , wherein the regulatory element is a 5′ or 3′ regulatory element.
10. The vector of claim 9 , wherein the 5′ regulatory element is a promoter.
11. The vector of claim 9 , wherein the 3′ regulatory element is a termination and poly-A addition sequence from the NOS gene of Agrobacterium tumefaciens.
12. A host cell containing the vector of claim 6 .
13. A cell culture comprising the host cell according to claim 12 .
14. A method of producing a transgenic plant cell, comprising transforming the plant cell with the nucleotide sequence of claim 1 .
15. The method of claim 14 , wherein the cell is transformed by direct uptake of at least one nucleotide sequence.
16. A method for the production of a protein for modulating embryogenesis or endosperm development, comprising cultivating the host cell of claim 12 in culture medium under conditions allowing the synthesis of the protein, and isolating the protein.
17. The vector of claim 10 , wherein the 5′ regulatory element is a CaMV 35S promoter.
18. The vector of claim 10 , wherein the 5′ regulatory element is a promoter located within the nucleotide sequence of SEQ ID NO: 14.
19. The host cell of claim 12 , which is a bacterial cell.
20. The host cell of claim 12 , which is a yeast cell.
21. The method of claim 14 , wherein the cell is transformed by Agrobacterium mediated transformation.
22. The method of claim 14 , wherein the cell is transformed by a method selected from the group of methods consisting of microinjection, electroporation, chemical treatment, and particle bombardment.
23. An isolated nucleotide sequence comprising the complementary sequence of SEQ ID NO: 4, 8, or 14.
24. A method of producing transgenic maize cell, comprising transforming the maize cell with a vector comprising the nucleotide sequence of claim 1 .
25. A method of producing transgenic maize plant, comprising transforming the plant with a vector comprising the nucleotide sequence of claim 1 .
26. The host cell of claim 12 , which is an egg cell from maize.
27. A plant comprising the vector of claim 10 .
28. Seed comprising the vector of claim 10 .