Sugar-regulatory sequences in alpha-amylase genes
View Patent ↗A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.
1. A chimeric plant promoter for upregulating expression of a coding sequence operatively linked to the promoter, under conditions of sugar depletion or deprivation in a plant cell, comprising:
(i) a promoter element effective to express the coding sequence in plant cells, under selected conditions, and
(ii) carried in said promoter element, a heterologous sequence from the Amy3D promoter comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 or combinations thereof.
2. The chimeric promoter of claim 1 , wherein the heterologous sequence is identified by SEQ ID NO: 1.
3. The chimeric promoter of claim 1 , wherein the heterologous sequence is identified by SEQ ID NO: 2.
4. The chimeric promoter of claim 1 , wherein the heterologous sequence is identified by SEQ ID NO: 3.
5. The chimeric promoter of claim 1 , wherein the heterologous sequence is identified by SEQ ID NO: 4.
6. The chimeric promoter of claim 1 , wherein the heterologous sequence is SEQ ID NO: 5.
7. The chimeric promoter of claim 1 , wherein the heterologous sequence is duplicated.
8. The chimeric promoter of claim 1 , wherein the promoter element is from a monocot α-amylase gene selected from the group consisting of the RAmy1A, RAmy1B, RAmy2A, RAmy3A, RAmy3B, RAmy3C, pM/C, gKAmy141, gKAmy155, Amy32b, and HV18 genes.
9. A method of enhancing the inducibility by sugar depletion or deprivation of a plant or plant-virus promoter in plant cells, said method comprising:
introducing into a plant promoter or plant-virus promoter, a heterologous sequence from the Amy3D promoter comprising SEQ ID NO:1, SEQ ID NO:2,SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO: 5 or combinations thereof,
by said introducing, achieving at least a 2-fold greater sugar-depletion induction level of the promoter in plant cells over that of the unmodified promoter.
10. The method of claim 9 , wherein the promoter element is from an α-amylase gene selected from the group consisting of the RAmy1A, RAmy1B, RAmy2A, RAmy3 A, RAmy3 B, RAmy3C, pM/C, gKAmy141,gKAmy155, Amy32b, and HV18 genes.
11. The method of claim 9 , wherein the heterologous sequence is duplicated in the chimeric promoter.
12. The method of claim 9 , wherein the plant promoter is from a monocot plant.
13. A vector for use in tranforming a plant, comprising:
a chimeric gene having, operatively linked in sequence in a 5′ to 3′ direction, (i) the chimeric plant promoter of claim 1 , (ii) a gene encoding a protein to be expressed, and (iii) a 3′ untranslated terminator region.
14. Plant cells transformed with the vector of claim 13 .
15. The plant cells of claim 14 , which are monocot cells.