System and method for transgenic and targeted mutagenesis screening
The present invention provides a method and apparatus for to conduct transgenic and targeted mutagenesis screening of genomic DNA. This invention also provides a system for screening DNA for a designated genetic sequence. The system includes a computer having a processor, memory and web browser, wherein the computer receives instructions concerning the designated genetic sequence and other screening parameter selection from a remote user via a form of electronic communication, and an automatic screening device that analyzes samples of genomic DNA for the designated sequence.
1. In a method for detecting a designated genetic sequence in a sample of murine genomic DNA, obtained from a lysed tissue sample, in a solid phase hybridization assay including a bipartite probe specific for a portion of said murine genomic DNA, the improvement comprising:
separating said murine genomic DNA from said lysed tissue sample using magnetic particles and immobilizing said murine genomic DNA on to a solid phase, wherein said bipartite probe has a first region specific for a portion of said murine genomic DNA and a second region that binds to a signal emitting dendrimer.
2. The method of claim 1 wherein said signal emitting dendrimer is cyanine 3.
3. The method of claim 1 wherein said probe specific for a portion of said murine genonlic DNA is selected from the group consisting of: SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, and SEQ ID NO. 25.
4. The method of claim 1 wherein said murine genomic DNA concentration is about 12.5 to 500 ng/μL.
5. The method of claim 1 wherein the murine genomic DNA concentration is 17 ng to 250 ng/μL.
6. The method of claim 1 wherein said solid phase is an optically flat substrate.
7. The method of claim 6 wherein said optically flat substrate is glass.