Gene 763 of phytopathogenic fungus
The invention concerns a novel nucleic acid fragment of the genome of rice pathogenic fungus Magnaporthe grisea comprising a gene coding for a protein (hereafter referred to as gene 763) whereof the presence and integrity are indispensable for pathogenesis of said fungus with respect to rice and barley. The invention also concerns the promoter of said gene, the gene coding for protein 763, protein 763 and uses thereof for identifying potential biological targets for novel fungicide molecules and for isolating genes coding for proteins controlling biochemical functions essential to the pathogenesis of the fungus Magnaporthe grisea with respect to rice and barley. The invention further concerns compounds inhibiting pathogenesis of fungi related to the expression of gene 763.
1. A polynucleotide, characterized in that it comprises a polynucleotide chosen from the following polynucleotides:
a) the polynucleotide of SEQ ID No. 1;
b) the polynucleotide of SEQ ID No. 2; and
c) the polynucleotide, the sequence of which is included between position 17 and position 733 of SEQ ID No. 2.
2. The polynucleotide as claimed in claim 1 , characterized in that it encodes a transcription factor which is functional in fungi.
3. The polynucleotide as claimed in claim 1 , characterized in that it encodes a transcription factor which is essential to fungal pathogenesis for plants.
4. A polynucleotide, characterized in that it comprises a polynucleotide encoding the polypeptide of SEQ ID No. 3.
5. A polynucleotide, characterized in that it comprises the promoter of gene 763, the sequence of which is included between position 1 and position 705 of SEQ ID No. 1.
6. An expression cassette, characterized in that it comprises, in the direction of transcription:
a) a promoter which is functional in a host organism;
b) a polynucleotide as claimed in claim 1 ; and
c) a sequence which is a terminator sequence in said host organism.
7. An expression cassette, characterized in that it comprises, in the direction of transcription:
a) a promoter which is functional in a host organism;
b) a polynucleotide encoding the polypeptide 763 or SEQ ID No. 3; and
c) a sequence which is a terminator sequence in said host organism.
8. An expression cassette, characterized in that it comprises, in the direction of transcription:
a) a polynucleotide as claimed in claim 5 ; and
b) a reporter gene; and
c) a terminator sequence.
9. A vector comprising a polynucleotide as claimed in claim 1 .
10. An isolated hot cell transformed with a polynucleotide as claimed in claim 1 .
11. A method for transforming isolated host cells by integrating into said isolated host cells at least one polynucleotide as claimed in claim 1 .
12. A vector comprising an expression cassette as claimed in claim 6 .
13. An isolated host cell transformed with an expression cassette as claimed in claim 6 .
14. An isolated host cell transformed with a vector as claimed in claim 9 .
15. A method for transforming isolated host cells by integrating into said isolated host cells an expression cassette as claimed in claim 6 .
16. A method for transforming isolated host cells by integrating into said isolated host cells a vector as claimed in claim 9 .