Randomized libraries of zinc finger proteins
The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes.
1. A nucleic acid library encoding randomized zinc finger proteins, wherein the library is generated by recognition helix grafting or by gene shuffling.
2. The library of claim 1 , wherein each member of the library further encodes a regulatory domain in operative linkage with the zinc finger protein.
3. The library of claim 2 , wherein the regulatory domain is an activation domain.
4. The library of claim 2 , wherein the regulatory domain is a repression domain.
5. A eukaryotic cell population transfected with the library of claim 1 .
6. The cell population of claim 5 , wherein the library is generated by recognition helix grafting.
7. The cell population of claim 5 , wherein the library is generated by gene shuffling.
8. The cell population of claim 5 , wherein each member of the library further encodes a regulatory domain in operative linkage with the zinc finger protein.
9. The cell population of claim 8 , wherein the regulatory domain is an activation domain.
10. The cell population of claim 8 , wherein the regulatory domain is a repression domain.