Polynucleotides encoding carnation senescense-induced EIF-5A
Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced eIF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.
1. An isolated DNA molecule encoding a carnation senescence-induced eIF-5A, wherein the DNA molecule has at least about 70% sequence identity with SEQ ID NO:13 and wherein the senescence-induced eIF-5A has translation initiation factor activity.
2. An isolated DNA molecule encoding senescence-induced eIF-5A wherein the DNA molecule comprises SEQ ID NO:13.
3. A vector comprising
(a) the isolated DNA molecule encoding senescence-induced eIF-5A of claim 1 ; and
(b) regulatory sequences operatively linked to the DNA molecule such that senescence-induced eIF-5A is expressed in a plant cell into which it is transformed.
4. A transgenic plant cell comprising the vector according to claim 3 .
5. A vector comprising
(a) the DNA molecule of claim 2 ; and (b) regulatory sequences operatively linked to the DNA molecule such that the senescence-induced eIF-5A is expressed in a plant cell into which it is transformed.
6. A transgenic plant cell comprising the vector according to claim 5 .