Polynucleotides encoding carnation senescence-induced DHS
Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced elF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced elF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.
1. An isolated DNA molecule encoding a carnation senescence-induced deoxyhypusine synthase.
2. The isolated DNA molecule of claim 1 , wherein said carnation senescence-induced deoxyhypusine synthase comprises SEQ ID NO:10.
3. An isolated DNA molecule encoding a carnation senescence-induced deoxyhypusine synthase, wherein the DNA molecule comprises a polynucleotide having at least about 70% sequence identity within SEQ ID NO:9.
4. A vector comprising the DNA molecule of claim 1 and regulatory sequences operatively linked to the DNA molecule such that the deoxyhypusine synthase is expressed in a carnation plant cell transformed with the vector to achieve co-suppression.
5. A method for inhibiting the expression of an endogenous senescence-induced deoxyhypusine synthase gene in a carnation plant cell, said method comprising
(1) transforming carnation plant cells with the vector of claim 4 and;
(2) growing said plant, whereby said DNA molecule is over-expressed and the endogenous senescence-induced deoxyhypusine synthase gene is inhibited by the expression of the DNA molecule encoding the exogenous senescence-induced deoxyhypusine synthase.
6. The method of claim 5 , wherein the regulatory sequences comprise a constitutive promoter.
7. A transgenic carnation plant cell comprising the vector of claim 4 .
8. A vector comprising the DNA molecule of claim 3 and regulatory sequences operatively linked to the DNA molecule such that the deoxyhypusine synthase is expressed in a carnation plant cell transformed with the vector to achieve co-suppression.
9. A method for inhibiting the expression of an endogenous senescence-induced deoxyhypusine synthase gene in a carnation plant by co-suppression, said method comprising
(1) transforming carnation plant cells with the vector of claim 8 ; and
(2) growing said plant, whereby said DNA molecule is over-expressed and the endogenous senescence-induced deoxyhypusine synthase gene is inhibited by the expression of the DNA molecule encoding the exogenous senescence-induced deoxyhypusine synthase.-
10. A transgenic carnation plant cell comprising the vector of claim 8 .
11. The method claim 9 wherein the regulatory sequences comprise a constitutive promoter.
12. The vector of claim 4 , wherein the senescence-induced deoxyhypusine synthase has the sequence set forth in SEQ ID NO:10.
13. The vector of claim 8 , wherein the DNA molecule comprises a polynucleotide having the sequence set forth in nucleotides 256-1374 of SEQ ID NO:9.
14. The method of any of claims 6 and 11 , wherein the constitutive promoter is a fig wart mosaic virus 35S promoter, a cauliflower mosaic virus (CaMV) 35S promoter, a double fig wart mosaic virus 35s promoter, a double CaMV 35S promoter, or a MAS promoter.