Acetylation of pRb by p300 assay method for compounds which modulate this process
View Patent ↗The present invention is based upon the finding that the protein p300 has acetylation activity which is directed to the retinoblastoma tumour suppressor protein pRb by the presence in the cell of the adenovirus E1A protein. This represents a target for modulators of the cell cycle, to which end the invention provides an assay for a modulator of acetylation of pRb by p300, which comprises: a) bringing into contact a p300 protein a pRb protein and a putative modulator compound under conditions where the p300 protein, in the absence of said modulator is capable of acetylating the pRb protein; b) providing conditions for acetylation of said pRb protein; and c) measuring the degree of inhibition of acetylation caused by said modulator compound.
1. An assay for a modulator of acetylation of pRb by p300, which comprises
a) bringing into contact a p300 protein, a pRb protein and a putative modulator compound under conditions where the p300 protein, in the absence of said modulator is capable of acetylating the pRb protein; and
b) providing conditions for acetylation of said pRb protein;
c) measuring the degree of inhibition of acetylation caused by said modulator compound.
2. An assay according to claim 1 wherein said pRb is in the form of a fusion protein with a detectable tag.
3. An assay according to claim 1 wherein the adenovirus E1A protein is present.
4. An assay according to claim 1 wherein the pRb is in the form of a C-terminal fragment of at least 40 amino acids comprising at least one target lysine residue.
5. An assay according to claim 1 wherein said p300 protein is a fragment of at least about 450 amino acids of a full-length p300, said fragment retaining the ability to acetylate pRb.
6. An assay according to claim 1 which is a cell based assay in which one or both of said p300 and pRb proteins are expressed using a recombinant DNA construct.
7. An assay according to claim 1 which further includes the step of phosphorylating the pRb protein using cyclinE/cdk2 as a means to determine the acetylation of pRb.