Rice catalase B promoter
View Patent ↗The present invention provides a rice catalase B promoter for inducing expression specifically in at least one site of roots and stem apices. By using this promoter, it is possible to synthesize a resistance substance against nematodes or soil pathogens in roots; synthesize a substance promoting the development of roots in the roots; produce a gene recombinant plant in which expression of a selective marker in the redifferentiated plant body is limited to roots when the plant is young, so as to suppress the expression when the plant is matured; or the like. Further, the capability of expression in stem apices can be used to develop a technique for suppress gene expression in vigorously proliferating cells.
1. An isolated promoter consisting of a nucleotide sequence indicated by SEQ ID No. 11.
2. A method for expressing a desired foreign coding sequence in a root or a stem apex of a plant, comprising the steps of:
(a) constructing a vector comprising the promoter of claim 1 operably linked to the desired foreign coding sequence; and
(b) introducing the vector to the root or the stem apex of the plant.
3. A method according to claim 2 , wherein the plant is a monocotyledon or a dicotyledon.
4. A method according to claim 2 , wherein the plant is a monocotyledon.
5. A method according to claim 2 , wherein the plant is rice, Arabidopsis , maize, wheat, barley, tomato, cucumber, eggplant, potato, lettuce, Japanese radish, or carrot.
6. A method according to claim 2 , wherein the desired foreign coding sequence is a coding sequence encoding at least one polypeptide selected from the group consisting of a polypeptide capable of conferring resistance to disease or nematodes or soil pathogens, a peptide capable of promoting development of roots, and a selective marker for a redifferentiated plant body.
7. A method according to claim 2 , wherein the desired foreign coding sequence is expressed in a tip portion of the roots.
8. A method according to claim 2 , wherein the desired foreign coding sequence is expressed in a meristem region of a young monocotyledon plant.
9. A method according to claim 2 , wherein the desired foreign coding sequence is expressed in a stem apex portion.