Universal collection medium
This invention provides a novel universal collection medium for cell collection. The medium allows for the first time the ability to perform cytology and direct molecular analysis on cells preserved in a single sample. This invention also provides novel methods for analyzing cells to assess human conditions.
1. A cell collection medium, wherein the cells contained in the medium can be analyzed directly by both cytological and molecular methods, wherein the molecular method of analysis comprises either RNA or DNA or protein analysis or the analysis of both RNA and DNA, wherein the medium is water based and comprises a buffer component, at least one alcohol, a non-crosslinking fixative and an agent to inhibit degradation of at least one of RNA, DNA, or protein, wherein the non-crosslinking fixative comprises about 1% to about 15% of the medium, and wherein the buffer component has a buffering capacity within a pH range of about 3 to about 5.
2. The medium of claim 1 , wherein the buffer component has a buffering capacity within a pH range of about 3.5 to about 4.5.
3. The medium of claim 1 , wherein the alcohol component comprises a C1 to C10 alcohol.
4. The medium of claim 3 , wherein the alcohol component is selected from the group consisting of methanol, ethanol, propanols, butanols, and pentanols.
5. The medium of claim 4 , wherein the alcohol component comprises ethanol or n-butanol.
6. The medium of claim 1 , wherein the fixative comprises a polymer selected from the group consisting of a poly(ethylene glycol) and a poly(ethylene oxide).
7. The medium of claim 1 , wherein the fixative is a poly(ethylene glycol).
8. The medium of claim 7 , wherein the poly(ethylene glycol) has a molecular weight of between about 600 and about 4600.
9. The medium of claim 8 , wherein the poly(ethylene glycol) has a molecular weight of about 1500.
10. The medium of claim 1 , wherein the agent to inhibit degradation of at least one of the group consisting of RNA, DNA, and protein comprises at least one agent selected from the group consisting of a nuclease inhibitor, a protease inhibitor and a chelating agent.
11. The medium of claim 10 , wherein the agent to inhibit degradation of at least one of the group consisting of RNA, DNA, and protein comprises a chelating agent.
12. The medium of claim 11 , wherein the chelating agent is selected from the group consisting of murexide, chromotropic acid, 1-(1-hydroxy-2-napththylazo-2-hydroxy-5-nitronaphthalene-4-sulphonic acid, ethylenediaminetetraacetic acid, o-phenanthroline, and thiourea.
13. The medium of claim 12 , wherein the chelating agent comprises ethylenediaminetetraacetic acid.
14. The medium of claim 1 , wherein the alcohol is selected from the group consisting of methanol, ethanol, propanols, butanols, and pentanols, wherein the agent to inhibit degradation is a chelating agent selected from the group consisting of murexide, chromotropic acid, 1-(1-hydroxy-2-napththylazo-2-hydroxy-5-nitronaphthalene-4-sulphonic acid, ethylenediaminetetraacetic acid, o-phenanthroline, and thiourea, and wherein the fixative comprises a polymer selected from the group consisting of a poly(ethylene glycol) and a poly(ethylene oxide).