Bioactive peptide for cell adhesion
The invention is directed to a polypeptide derived from domain IV of the extracellular matrix protein perlecan that can selectively adhere cells, nucleic acids encoding the inventive polypeptide, vectors comprising the nucleic acids, devices comprising a scaffold coated with the inventive polypeptide, and methods of adhering cells to a scaffold using the inventive polypeptide.
1. An isolated polypeptide consisting of SEQ ID NO: 1, wherein the polypeptide has cell adhesion activity.
2. The isolated polypeptide of claim 1 , wherein the polypeptide adheres to a cell from at least one cell line selected from the group consisting of HES, PC3 and 10 T½ cell lines.
3. A composition comprising the isolated polypeptide of claim 1 and a carrier.
4. The composition of claim 3 , wherein the carrier is bovine serum albumin, fibronectin, or collagen.
5. A method for adhering eukaryotic cells to a scaffold comprising:
(a) applying the isolated polypeptide of claim 1 to the scaffold; and
(b) adhering the cells to the scaffold.
6. The method of claim 5 , wherein the isolated polypeptide consists of the amino acid sequence of SEQ ID NO: 1.
7. The method of claim 5 , wherein the scaffold is a medical device.
8. The method of claim 7 , wherein the medical device is selected from the group consisting of a prosthesis and a stent.
9. The method of claim 5 , wherein the cell is selected from the group consisting of bone cell precursors and cartilage cell precursors.