Translucent solid matrix assay device for microarray analysis
The present invention concerns methods and compositions relating to matrix arrays. In certain embodiments, the arrays are translucent. In other embodiments, the arrays are reconfigurable. In preferred embodiments, the arrays are translucent and reconfigurable. Reconfigurable arrays may be produced using small linker molecules, such as aptamers or affibodies, attached to the array substrate. Preferably, the small linker molecules bind to an IgG specific portion of antibodies. Such arrays may be used to detect any target that binds selectively or specifically to an IgG, allowing great flexibility of use. Translucent matrix arrays may utilize a translucent, colloidal form of nitrocellulose to coat the array substrate.
1. A translucent matrix array comprising:
a) a substrate;
b) an optically clear layer of colloidal nitrocellulose coated on the substrate; and
c) one or more first probes attached to the colloidal nitrocellulose.
2. The translucent matrix array of claim 1 , wherein the first probes are attached to the array in spots of about 300 μm in diameter.
3. The translucent matrix array of claim 2 , wherein the probes attached to different spots on the array bind to different targets.
4. The translucent matrix array of claim 1 , wherein the first probes are selected from the group consisting of antibodies, antibody fragments, FAb fragments, humanized antibodies, single-chain antibodies, chimeric antibodies, oligonucleotides, polynucleotides, nucleic acids, aptamers and affibodies.
5. The translucent matrix array of claim 1 , wherein the substrate is a glass substrate.
6. The translucent matrix array of claim 1 , wherein the substrate is a plastic substrate.
7. The translucent matrix array of claim 4 , wherein the first probes are aptamers or affibodies.
8. The translucent matrix array of claim 7 , wherein the first probes bind to antibodies, antibody fragments, FAb fragments, humanized antibodies, single-chain antibodies or chimeric antibodies.
9. The translucent matrix array of claim 8 , wherein the first probes bind to IgG (immunoglobulin type G) antibodies.
10. The translucent matrix array of claim 4 , wherein the first probes are antibodies.
11. The translucent matrix array of claim 10 , wherein the first probes are anti-mouse antibodies.
12. The translucent matrix array of claim 1 , wherein the first probes are attached directly to the nitrocellulose.
13. The translucent matrix array of claim 1 , wherein the one or more first probes are capable of binding to one or more targets.
14. The translucent matrix array of claim 13 , wherein the one or more targets are selected from the group consisting of a protein, peptide, carbohydrate, polysaccharide, glycoprotein, lipid, hormone, growth factor, cytokine, receptor, antigen, allergen, antibody, substrate, metabolite, cofactor, inhibitor, drug, pharmaceutical, nutrient, toxin, poison, explosive, pesticide, chemical warfare agent, biowarfare agent, biohazardous agent, infectious agent, prion, radioisotope, vitamin, heterocyclic aromatic compound, carcinogen, mutagen, narcotic, amphetamine, barbiturate, hallucinogen, waste product, contaminant, heavy metal, virus, bacterium, Salmonella, Streptococcus, Legionella, E. coli, Giardia, Cryptosporidium, Rickettsia , spore, mold, yeast, algae, amoebae, dinoflagellate, unicellular organism, pathogen and cell.
15. The translucent matrix array of claim 13 , further comprising an optical detector aligned to detect fluorescent labels attached to the targets.
16. The translucent matrix array of claim 15 , wherein the optical detector comprises a CMOS imager.
17. The translucent matrix array of claim 1 , wherein the optically clear nitrocellulose layer transmits light signals without scatter or interference.