Spinocerebellar ataxia type 8 and methods of detection
The present invention provides an isolated nucleic acid molecule containing a repeat region of an isolated spinocerebellar ataxia type 8 (SCA8) coding sequence, the coding sequence located within the long arm of chromosome 13, and the complement of the nucleic acid molecule. Diagnostic methods based on identification of this repeat region are also provided.
1. An isolated nucleic acid molecule comprising:
a) nucleotides 1-448 of SEQ ID NO:1 followed by a repeat region, or
b) nucleotides 726-1,159 of SEQ ID NO:1 preceded by a repeat region, or
c) a sequence which is exactly complementary to the full length of a) or b),
wherein the repeat region comprises a (CTG)n repeat wherein n is at least about 80 or a combined ((CTG)/(CTA))n repeat wherein n is at least about 92;
wherein the nucleic acid molecule is capable of hybridizing to a portion of SEQ ID NO:1 or a complement of a portion of SEQ ID NO:1.
2. The isolated nucleic acid molecule of claim 1 wherein the nucleic acid molecule comprises DNA.
3. The DNA of claim 2 wherein the DNA is cDNA.
4. The isolated nucleic acid molecule of claim 1 wherein the nucleic acid molecule comprises nucleotides 1-448 of SEQ ID NO:1 followed by a repeat region followed by nucleotides 726-1,159 of SEQ ID NO:1, or the sequence which is exactly complementary thereto, wherein the repeat region comprises a (CTG)n repeat wherein n is at least about 80 or a combined ((CTG)/(CTA))n repeat wherein n is at least about 92.
5. An isolated nucleic acid molecule wherein the nucleic acid molecule consists of nucleotides 1-448 of SEQ ID NO:1, or the sequence which is exactly complementary over the full length of nucleotides 1-448 of SEQ ID NO:1 thereto.
6. An isolated nucleic acid molecule wherein the nucleic acid molecule consists of nucleotides 726-1,159 of SEQ ID NO:1, or the sequence which is exactly complementary over the full length of nucleotides 726-1,159 of SEQ ID NO:1 thereto.
7. An isolated nucleic acid molecule comprising nucleotides 1-448 of SEQ ID NO:1 in a vector;
wherein the nucleic acid molecule is capable of hybridizing to a portion of SEQ ID NO:1 or a complement of a portion of SEQ ID NO:1.
8. An isolated nucleic acid molecule comprising nucleotides 1-448 of SEQ ID NO:1 and further comprising a repeat region, or the sequence which is exactly complementary over the full length of nucleotides 1-448 of SEQ ID NO:1 thereto, wherein the repeat region comprises a (CTG)n repeat wherein n is at least about 80 or a combined ((CTG)/(CTA))n repeat wherein n is at least about 92;
wherein the nucleic acid molecule is capable of hybridizing to a portion of SEQ ID NO:1 or a complement of a portion of SEQ ID NO:1.
9. An isolated oligonucleotide consisting of at least 15 consecutive nucleotides and no more than about 35 consecutive nucleotides from nucleotides 1-448 of SEQ ID NO:1,or the sequence which is exactly complementary over the full length of the at least 15 consecutive nucleotides to no more than about 35 consecutive nucleotides from nucleotides 1-448 of SEQ ID NO:1 thereto.
10. An isolated oligonucleotide consisting of at least 15 consecutive nucleotides and no more than about 35 nucleotides from nucleotides 726-1,159 of SEQ ID NO: 1,or the sequence which is exactly complementary over the full length of the at least 15 consecutive nucleotides to no more than about 35 consecutive nucleotides from nucleotides 726-1,159 of SEQ ID NO:1 thereto.
11. An isolated recombinant vector comprising the nucleotides 1-448 of SEQ ID NO:1 followed by a repeat region followed by nucleotides 726-1,159 of SEQ ID NO:1 operatively linked to heterologous vector sequences;
wherein the nucleic acid molecule is capable of hybridizing to a portion of SEQ ID NO:1 or a complement of a portion of SEQ ID NO:1.
12. An isolated nucleic acid molecule selected from the group consisting of:
a) DNA consisting of SEQ ID NO: 1, or the sequence which is exactly complementary over the full length of SEQ ID NO:1 thereto;
b) RNA transcribed from the DNA of a); and
c) a fragment of the DNA of a) or RNA of b),
wherein the fragment consists of at least 15 nucleotides and no more than about 35 nucleotides of either nucleotides 1-448 or nucleotides 726-1159 of SEQ ID NO: 1 or is exactly complementary over the full length of at least 15 nucleotides and no more than about 35 nucleotides of either nucleotides 1-448 or nucleotides 726-1159 of SEQ ID NO: 1.
13. An isolated nucleic acid primer selected from the group consisting of SEQ ID NO: 4, 5, 8, 9 and 12.
14. A nucleic acid probe consisting of nucleotides 19-449 of SEQ ID NO:1, or the sequence which is exactly complementary over the full length of nucleotides 19-449 of SEQ ID NO:1 thereto.
15. An isolated nucleic acid molecule consisting of:
a) nucleotides 1-448 of SEQ ID NO:1;
a repeat region following nucleotides 1-448 of SEQ ID NO:1, wherein the repeat region comprises either a (CTG)n repeat wherein n is at least about 80 or a combined ((CTG)/(CTA))n repeat wherein n is at least about 92; and
nucleotides 726-1159 of SEQ ID NO:1 following the repeat region; or
b) the nucleic acid molecule having the nucleotide sequence exactly complementary to the full length of the nucleic acid molecule of a).
16. The isolated nucleic molecule of claim 15 wherein the (CTG) repeat n is less than 700 and the ((CTG)/(CTA)) repeat n is less than 179.
17. An isolated nucleic acid molecule consisting of:
a) nucleotides 1-448 of SEQ ID NO:1;
a repeat region following nucleotides 1-448 of SEQ ID NO:1,wherein the repeat region comprises either a (CTG)n repeat wherein n is less than 80 or a combined ((CTG)/(CTA))n repeat wherein n is less than 91; and
nucleotides 726-1159 of SEQ ID NO:1 following the repeat region; or
b) the nucleic acid molecule having the nucleotide sequence exactly complementary to the full length of the nucleic acid molecule of a).