Device for removal of prions from blood, plasma and other liquids
View Patent ↗Devices such as flow through columns, substrates such as spherical polymer beads, and methods of using such to remove prions from any liquid sample are disclosed. A surface of a substrate is coated with a prion complexing agent, such as a salt of phosphotungstic acid. Blood or plasma passing through a column containing beads coated with prion complexing agent are rendered prion free.
1. A device for removing infectious PrP Sc dimers from a liquid sample, comprising:
a housing through which a liquid sample flows;
substrate surfaces comprised of a heteropoly acid or salt thereof; and
infectious PrP Sc dimers bound to the heteropoly acid or salt thereof.
2. The device of claim 1 , wherein the housing is cylindrical.
3. The device of claim 1 , wherein the substrate is comprised of polymer beads having the heteropoly acid or salt thereof coated on their surface.
4. The device of claim 1 , wherein the heteropoly acid or salt thereof is a phosphoptungstic acid or salt thereof.
5. The device of claim 4 , wherein the phosphotungstic acid or salt thereof is sodium phosphotungstate.
6. A device for removing infectious PrP Sc dimers from a liquid sample, comprising:
a housing through which a liquid sample flow;
substrate surfaces having thereon a complexing agent which binds infectious PrP Sc dimers; and
infectious PrP Sc dimers bound to the complexing agent.
7. The device of claim 6 , wherein the complexing agent is comprised of an antibody which binds PrP Sc .
8. The device as claimed in claim 6 , wherein the complexing agent is a heteropoly acid or salt thereof.
9. The device as claimed in claim 8 , wherein the heteropoly acid or salt thereof is a phosphotungstic acid or salt thereof.
10. The device as claimed in claim 9 , wherein the phosphotungstic acid or salt thereof is sodium phosphotungstate.