Rf2a and rf2b transcription factors
A method of activating the rice tungro bacilliform virus (RTBV) promoter in vivo is disclosed. The RTBV promoter is activated by exposure to at least one protein selected from the group consisting of Rf2a and Rf2b.
1. A method of achieving constitutive expression of a gene of interest in plant cells, which comprises:
(i) transforming the plant cells with a first nucleic acid sequence that comprises the gene of interest operably linked to a RTBV promoter,
(ii) transforming the plant cells with a second nucleic acid sequence conferring the constitutive expression of (SEQ ID NO: 1) the coding sequence for Rf2a, and
(iii) growing the plant cells, wherein said constitutive expression of the gene of interest is achieved in the plant cells.
2. The method of claim 1 , wherein the plant cells are not rice cells.
3. A plant transformation vector, capable of transforming plant cells to achieve constitutive expression of a gene of interest, comprising (i) a RTBV promoter operably linked to the gene of interest and (ii) a constitutive promoter operably linked to a Rf2a encoding sequence.
4. The vector of claim 3 , wherein the constitutive promoter is a CaMV 35S promoter.
5. The vector of claim 3 , wherein the plant cells are not rice cells.
6. A set of plant transformation vectors, capable of transforming plant cells to achieve constitutive expression of a gene of interest, comprising (i) a first vector comprising a RTBV promoter operably linked to the gene of interest and (ii) a second vector comprising a constitutive promoter operably linked to a Rf2a encoding sequence.
7. The vector of claim 6 , wherein the constitutive promoter is a CaMV 35S promoter.
8. The vector of claim 6 , wherein the plant cells are not rice cells.