Recombinant immunogenic compositions and methods for protecting against lethal infections from
View Patent ↗Recombinant immunogenic compositions and methods for protecting against lethal infections from Bacillus anthracis having a variant of recombinant Bacillus anthracis protective antigen (rPA) and a variant of recombinant Bacillus anthracis lethal factor (rLF). These proteins may be expressed separately or as a fusion protein. The recombinant proteins are produced in an avirulent strain of Bacillus anthracis that overproduces the desired antigens. The compositions and methods induce the animal host to produce antibodies against a virulent strain of Bacillus anthracis.
1. An immunogenic composition comprising a recombinant B. anthracis PA (rPA) protein comprising the amino acid sequence set forth in SEQ ID NO: 9 and a recombinant B. anthracis LF (rLF) protein comprising the amino acid sequence set forth in SEQ ID NO: 6.
2. The composition of claim 1 , further comprising at least one adjuvant.
3. The immunogenic composition of claim 2 , wherein said adjuvant is aluminum hydroxide, an immunostimulatory sequence (ISS), CpG, or calcium phosphate.
4. A method of producing an immunogenic response in an animal comprising the step of: administering to an animal the immunogenic composition of claim 1 .
5. The method of claim 4 , wherein the composition comprises rPA to rLF at a ratio of 5:2.
6. The method of claim 4 , further comprising the step of administering at least one adjuvant to the animal.
7. The method of claim 5 , wherein said adjuvant is aluminum hydroxide, an immunostimulatory sequence (ISS), CpG, or calcium phosphate.
8. The method of claim 7 , wherein the composition comprises 50 μg rPA and 20 μg rLF per 0.5 ml of the aluminum hydroxide.