Gene shinc-3 and diagnostic and therapeutic uses thereof
The invention provides a SHINC-3 polynucleotide, which can be a nucleic acid encoding all or a portion of a SHINC-3 protein, or a complementary polynucleotide or antisense polynucleotide. In another aspect, the invention provides a SHINC-3 polypeptide, which can be a full-length SHINC-3 protein or a fragment thereof or an analog or homolog thereof. Desirably, the SHINC-3 polypeptide modulates apoptosis. In another aspect, the invention provides an antibody that specifically binds a SHINC-3 polypeptide. In another aspect, the invention provides diagnostic methods. For example, the method affords a method for identifying compounds that modulate apoptosis. In another aspect, the invention provides a method for detecting or evaluating the prognosis of a cancer. In another aspect, the invention provides diagnostic compositions for detection of cancer. In another aspect, the invention provides a method of modulating apoptosis or invention or preventing a cancer, tumor growth and/or metastasis by administration of an agent that modulates the expression and/or activity of SHINC-3. In another aspect, the invention provides formulations of SHINC-3 polynucleotides or proteins. Preferably, such compositions will comprise liposomal formulations.
1. An isolated nucleic acid molecular comprising a polynucleotide selected from the group consisting of
(a) a polynucleotide comprising nucleotides 1 to 2684 of SEQ ID NO: 2
(b) a polynucleotide comprising nucleotides 2 to 2684 of SEQ ID NO: 2, and
(c) a complete polynucleotide complement of the polynucleotide of (a) or (b).
2. The isolated nucleic acid of claim 1 , which is DNA.
3. A method of making a recombinant vector comprising inserting a nucleic acid molecule of claim 1 into a vector in operable linkage to a promoter.
4. A recombinant vector produced by the method of claim 3 .
5. The recombinant vector of claim 4 , wherein the vector further comprises an expression cassette.
6. A method of making a recombinant host cell comprising introducing the recombinant vector of claim 4 or 5 into a host cell.
7. A recombinant host cell produced by the method of claim 6 .
8. A recombinant method of producing a polypeptide, comprising culturing the recombinant host cell of claim 7 under conditions such that said polypeptide is expressed and recovering said polypeptide.