Nucleic acids and proteins from
The invention provides proteins from group B streptococcus ( Streptococcus agalactiae ) and group A streptococcus ( Streptococcus pyogenes ), including amino acid sequences and the corresponding nucleotide sequences. Data are given to show that the proteins are useful antigens for vaccines, immunogenic compositions, and/or diagnostics. The proteins are also targets for antibiotics.
1. A recombinant expression cassette comprising an isolated nucleic acid molecule which encodes a protein which comprises the amino acid sequence shown in SEQ ID NO:8780.
2. The recombinant expression cassette of claim 1 which comprises SEQ ID NO:8779.
3. A recombinant expression cassette comprising an isolated nucleic acid molecule which encodes a polypeptide with at least 85% amino acid sequence identity to SEQ ID NO:8780.
4. The recombinant expression cassette of claim 3 wherein the amino acid sequence has at least 90% sequence identity to SEQ ID NO:8780.
5. The recombinant expression cassette of claim 3 wherein the amino acid sequence has at least 95% sequence identity to SEQ ID NO:8780.
6. The recombinant expression cassette of claim 3 wherein the amino acid sequence has at least 99% sequence identity to SEQ ID NO:8780.
7. A composition comprising an isolated protein which comprises the amino acid sequence shown in SEQ ID NO:8780.
8. The composition of claim 7 , further comprising one or more of the following antigens:
a protein antigen from Helicobacter pylori;
a protein antigen from N. meningitidis serogroup B;
an outer-membrane vesicle (OMV) preparation from N. meningitidis serogroup B;
a saccharide antigen from N. meningitidis serogroup A, C, W135 and/or Y;
a saccharide antigen from Streptococcus pneumoniae;
an antigen from hepatitis A virus;
an antigen from hepatitis B virus;
a diphtheria antigen;
a tetanus antigen;
a saccharide antigen from Haemophilus influenzae B;
an antigen from N. gonorrhoeae;
an antigen from Chlamydia pneumoniae;
an antigen from Chlamydia trachomatis;
an antigen from Aporphyromonas gingivalis;
a polio antigen;
a rabies antigen;
an antigen from Moraxella catarrhalis ; and/or
an antigen from Staphylococcus aureus.
9. The composition of claim 7 further comprising an adjuvant.
10. The composition of claim 7 which is a diagnostic composition.
11. The composition of claim 9 wherein the adjuvant is Freund's adjuvant.
12. The composition of claim 7 further comprising a carbohydrate antigen.
13. The composition of claim 7 further comprising a saccharide antigen.
14. A composition comprising two or more isolated proteins, wherein one of the two proteins comprises the amino acid sequence shown in SEQ ID NO:3922 and the other of the two proteins comprises the amino acid sequence shown in SEQ ID NO:8780.
15. A composition comprising an isolated polypeptide which comprises an amino acid sequence with at least 85% amino acid sequence identity to SEQ ID NO:8780.
16. The composition of claim 15 wherein the amino acid sequence has at least 90% sequence identity to SEQ ID NO:8780.
17. The composition of claim 15 wherein the amino acid sequence has at least 95% sequence identity to SEQ ID NO:8780.
18. The composition of claim 15 wherein the amino acid sequence has at least 99% sequence identity to SEQ ID NO:8780.
19. The composition of claim 15 further comprising one or more of the one or more of the following antigens:
a protein antigen from Helicobacter pylori;
a protein antigen from N. meningitidis serogroup B;
an outer-membrane vesicle (OMV) preparation from N. meningitidis serogroup B;
a saccharide antigen from N. meningitidis serogroup A, C, W135 and/or Y;
a saccharide antigen from Streptococcus pneumoniae;
an antigen from hepatitis A virus;
an antigen from hepatitis B virus;
a diphtheria antigen;
a tetanus antigen;
a saccharide antigen from Haemophilus influenzae B;
an antigen from N. gonorrhoeae;
an antigen from Chlamydia pneumoniae;
an antigen from Chlamydia trachomatis;
an antigen from Aporphyromonas gingivalis;
a polio antigen;
a rabies antigen;
an antigen from Moraxella catarrhalis ; and/or
an antigen from Staphylococcus aureus.
20. The composition of claim 15 further comprising an adjuvant.
21. The composition of claim 15 which is a diagnostic composition.
22. The composition of claim 15 further comprising a carbohydrate antigen.
23. The composition of claim 15 further comprising a saccharide antigen.
24. The composition of claim 20 wherein the adjuvant is Freund's adjuvant.
25. A purified protein comprising the amino acid sequence shown in SEQ ID NO:8780.
26. A purified protein, wherein the amino acid sequence of the purified protein is at least 85% identical to the amino acid sequence shown in SEQ ID NO:8780.
27. The purified protein of claim 26 wherein the amino acid sequence is at least 90% identical to the amino acid sequence shown in SEQ ID NO:8780.
28. The purified protein of claim 26 wherein the amino acid sequence is at least 95% identical to the amino acid sequence shown in SEQ ID NO:8780.
29. The purified protein of claim 26 wherein the amino acid sequence is at least 99% identical to the amino acid sequence shown in SEQ ID NO:8780.
30. A method of raising an immune response in a patient against a group B Streptococcus which comprises a protein comprising the amino acid sequence SEQ ID NO:8780, comprising administering to the patient an effective amount of a composition comprising (i) an isolated protein comprising the amino acid sequence SEQ ID NO:8780 and (ii) an adjuvant.
31. The method of claim 30 wherein the group B Streptococcus is serotype III COH1.
32. The method of claim 30 wherein the group B Streptococcus is serotype V 2603 V/R.
33. The method of claim 30 wherein the adjuvant is Freund's adjuvant.
34. A method of raising an immune response in a patient against a group B Streptococcus which comprises a protein comprising the amino acid sequence SEQ ID NO:8780, comprising administering to the patient an effective amount of a composition comprising (i) a purified protein comprising an amino acid sequence which is at least 85% identical to the amino acid sequence shown in SEQ ID NO:8780 and (ii) an adjuvant.
35. The method of claim 34 wherein the amino acid sequence is at least 90% identical to the amino acid sequence shown in SEQ ID NO:8780.
36. The method of claim 34 wherein the amino acid sequence is at least 95% identical to the amino acid sequence shown in SEQ ID NO:8780.
37. The method of claim 34 wherein the amino acid sequence is at least 99% identical to the amino acid sequence shown in SEQ ID NO:8780.
38. The method of claim 34 wherein the group B Streptococcus is serotype III COH1.
39. The method of claim 34 wherein the group B Streptococcus is serotype V 2603 V/R.
40. The method of claim 34 wherein the adjuvant is Freund's adjuvant.