Compositions and methods for using a solid support to purify RNA
Reagents, methods and kits for the purification of RNA from biological materials are provided.
1. A formulation for isolating and purifying nucleic acids comprising:
a lithium salt at a concentration of about 4–10 M, a detergent, and a buffer, wherein the formulation has a pH above about 7 and lacks a chaotrope.
2. The formulation of claim 1 , further comprising a chelating agent.
3. The formulation of claim 1 , further comprising a reducing agent.
4. The formulation of claim 1 , wherein the lithium salt is lithium chloride.
5. The formulation of claim 1 , wherein the solution has a pH between about 7 and about 9.
6. The formulation of claim 1 , wherein the detergent is an anionic, cationic, zwitterionic or non-ionic detergent.
7. The formulation of claim 6 , wherein the detergent is a non-ionic detergent.
8. The formulation of claim 7 , wherein the detergent is a tween, triton, nonidet, igepal or tergitol.
9. The formulation of claim 1 , wherein the detergent is a mixture of triton-x and diethyl glycol monoethyl ether (DGME).
10. The formulation of claim 1 , wherein the detergent is a mixture of 5% v/v triton-x and 5% v/v DGME.
11. The formulation of claim 1 , wherein the detergent is present at a concentration of about 10% v/v of the final volume of the solution.
12. The formulation of claim 1 , wherein the buffer has a pKa of at least about 8.
13. The formulation of claim 1 , wherein the chelating agent is EDTA.
14. The formulation of claim 1 , wherein the reducing agent is Tris (carboxyethyl) phosphine (TCEP).
15. The formulation of claim 1 , further comprising a tungstate.
16. An article of manufacture for isolating and purifying nucleic acids comprising:
the formulation of claim 1 ; and
a solid support.
17. The formulation of claim 1 , further comprising a surfactant.
18. The formulation of claim 17 , wherein the surfactant is DGME.
19. A formulation for isolating and purifying nucleic acids comprising:
a lithium salt at a concentration of about 4–10 M, a detergent, and a buffer, wherein the formulation has a pH below about 4.5 and lacks a chaotrope.
20. An article of manufacture for isolating and purifying nucleic acids, comprising:
the formulation of claim 19 ; and
a solid support.