Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
View Patent ↗The present invention relates to recombinant nucleic acid molecules which contain two or more nucleotide sequences which encode enzymes which participate in the starch metabolism, methods for generating transgenic plant cells and plants which synthesize starch which is modified with regard to its phosphate content and its side-chain structure. The present invention furthermore relates to vectors and host cells which contain the nucleic acid molecules according to the invention, the plant cells and plants which originate from the methods according to the invention, to the starch synthesized by the plant cells and plants according to the invention, and to processes for the preparation of this starch.
1. An isolated recombinant nucleic acid molecule comprising:
(a) at least one fragment of a sequence encoding a potato soluble starch synthase III; and
(b) at least one fragment of a sequence encoding a potato branching enzyme, wherein the fragments are at least 500 nucleotides in length, and wherein the nucleic acid molecule inhibits synthesis of endogenous soluble starch synthase III and endogenous branching enzyme when introduced into plants.
2. The nucleic acid molecule of claim 1 , which is a deoxyribonucleic acid molecule.
3. The nucleic acid molecule of claim 1 , which is a ribonucleic acid molecule.
4. The nucleic acid molecule of claim 1 , wherein the synthesis of endogenous soluble starch synthase III and endogenous branching enzyme is inhibited by cosuppression.
5. The nucleic acid molecule of claim 1 , wherein the synthesis of endogenous soluble starch synthase III and endogenous branching enzyme is inhibited by antisense.
6. A vector comprising the nucleic acid molecule of claim 1 .
7. The vector according to claim 6 , wherein the nucleotide sequence encoding the fragment of a potato soluble starch synthase III is present in antisense orientation.
8. The vector according to claim 6 , wherein the nucleotide sequence encoding the fragment of a potato branching enzyme is present in antisense orientation.
9. The vector according to claim 6 , wherein the nucleotide sequence encoding the fragment of a potato soluble starch synthase III is present in sense orientation.
10. The vector according to claim 6 , wherein the nucleotide sequence encoding the fragment of a potato branching enzyme is present in sense orientation.
11. The vector according to claim 6 , wherein the nucleic acid molecule is linked to one or more regulatory elements which ensure transcription and synthesis of an RNA in a eukaryotic cell.
12. A transgenic plant cell, comprising the nucleic acid molecule of claim 1 , wherein the plant cell is a potato plant cell.
13. A method of generating a transgenic potato plant which synthesizes a modified starch, comprising regenerating an entire plant from the plant cell of claim 12 .
14. A transgenic potato plant obtained by the method of claim 13 .
15. Propagation material of the plant of claim 14 , wherein the propagation material contains the plant cell.
16. A method for the preparation of a transgenic plant cell comprising transfecting the plant cell with the nucleic molecule of claim 1 , wherein the plant cell is a potato plant cell.
17. A method of generating a transgenic plant cell comprising simultaneously or subsequently integrating into the genome of said plant cell:
(a) at least one fragment of a sequence encoding a potato soluble starch synthase III; and
(b) at least one fragment of a sequence encoding a potato branching enzyme thereby inhibiting the synthesis of endogenous soluble starch synthase III and endogenous branching enzyme,
wherein the plant cell is a potato plant cell, and
wherein said fragments are at least 500 nucleotides in length.
18. A method for inhibiting synthesis of endogenous soluble starch synthase III and endogenous branching enzyme in a potato plant comprising transforming the plant with the nucleic acid molecule of claim 1 , whereby the synthesis of endogenous soluble starch synthase III and endogenous branching enzyme is inhibited by cosuppression or antisense.
19. A method for producing starch comprising obtaining starch from the plant cell of claim 12 .
20. A method for producing starch comprising obtaining starch from the plant of claim 14 .
21. A method for producing starch comprising obtaining starch from the propagation material of claim 15 .
22. The nucleic acid molecule of claim 1 , wherein the potato branching enzyme is the potato branching enzyme I (BE I).
23. The method of claim 17 , wherein the potato branching enzyme is the potato branching enzyme I (BE I).