Tissue specific promoters
The invention relates to tissue specific promoters which can be used in plants for one or more of the following purposes: a. altering carbohydrate metabolism b. preventing memory substance loss c. expression of an invertase inhibitor d. expression of a fructosyl transferase e. expression of a levan sucrase f. expression of genes coding for transported proteins for N-compounds g. expression of characteristics which increase resistance/tolerance with respect to pathogens.
1. An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 1;
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1;
c. a sequence having root-specific promoter activity that hybridizes under stringent hybridizing conditions to SEQ ID NO: 1; and
d. a sequence that hybridizes under stringent hybridizing conditions to a sequence complementary to the full length of SEQ ID NO: 1, wherein said sequence that hybridizes has root-specific promoter activity;
wherein the stringent hybridizing conditions are either:
(1) hybridizing in 4×SSC at 65° C and subsequent multiple washing in 0.1×SSC at 65° C. for a total of 1 hour; or
(2) hybridizing at 68° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2×SSC and 0.1% SDS at 68° C.
2. The isolated promoter of claim 1 inserted into a vector or operably connected to a mobile genetic element.
3. A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 1 having a promoter activity;
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1;
c. a sequence having root-specific promoter activity that hybridizes under stringent hybridizing conditions to SEQ ID NO: 1; and
d. a sequence that hybridizes under stringent hybridizing conditions to a sequence complementary to the full length of SEQ ID NO: 1, wherein said sequence that hybridizes has root-specific promoter activity;
wherein the stringent hybridizing conditions are either:
(1) hybridizing in 4×SSC at 65° C. and subsequent multiple washing in 0.1×SSC at 65° C. for a total of 1 hour; or
(2) hybridizing at 68° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2×SSC and 0.1% SDS at 68° C.
4. A transgenic plant comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 1 having a promoter activity;
b. a sequence complementary to the full length of SEQ ID NO: 1;
c. the polynucleotide sequence having root-specific promoter activity that hybridizes under stringent hybridizing conditions to SEQ ID NO: 1; and
d. a sequence that hybridizes under stringent hybridizing conditions to a sequence complementary to the full length of SEQ ID NO: 1, wherein said sequence that hybridizes has root-specific promoter activity;
wherein the stringent hybridizing conditions are either:
(1) hybridizing in 4×SSC at 65° C. and subsequent multiple washing in 0.1×SSC at 65° C. for a total of 1 hour; or
(2) hybridizing at 68° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2×SSC and 0.1% SDS at 68° C.
5. The transgenic plant according to claim 4 , wherein said transgenic plant is Beta vulgaris , and wherein said promoter is active in the root, but not the above ground organs, of Beta vulgaris.
6. The transgenic plant according to claim 4 , wherein said transgenic plant is further characterized by the expression of a transgene exclusively:
a. in at least one of an above-ground or chloroplast containing plant part, or
b. in a below-ground organ,
wherein said expression is obtainable by transformation of a plant cell with said promoter that is operably linked to a transgene.
7. A transgenic seed produced by said transgenic plant of claim 4 .
8. The transgenic plant according to claim 6 , wherein said below-ground organ is a root.
9. The transgenic plant according to claim 6 , wherein said plant belongs to species beta vuigaris , and wnerein said promoter is active in the root, but not the above ground organs, of Beta vulgaris .
10. The transgenic plant according to claim 6 , wherein said transgenic plant is characterized by:
a. amending a carbohydrate metabolism;
b. avoiding a loss of a storage substance;
c. expressing an invertase inhibitor;
d. expressing a fructosyl transferase;
e. expressing a levan sucrase;
f. expressing a gene coding for a transporter protein for a N-compound; or
g. developing a feature that increases at least one of resistance and tolerance towards pathogens.
11. A method of:
(a) amending carbohydrate metabolism,
(b) avoiding loss of storage substance,
(c) increasing at least one of resistance and tolerance towards a pathogen, or
(d) expressing from a transgene:
an invertase inhibitor,
a fructosyl transferase,
a levan sucrase, or
a transporter protein for a N-compound, in a transgenic plant,
said method comprising:
a. transforming a plant cell with a promoter, wherein said promoter is operably linked to said transgene, and
b. subsequently regenerating a transgenic plant from said plant cell,
wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
i. SEQ ID NO: 1;
ii. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1;
iii. a sequence having root-specific promoter activity that hybridizes under stringent hybridizing conditions to SEQ ID NO: 1; and
iv. a sequence that hybridizes under stringent hybridizing conditions to a sequence complementary to the full length of SEQ ID NO: 1, wherein said sequence that hybridizes has root-specific promoter activity;
wherein the stringent hybridizing conditions are either:
(1) hybridizing in 4×SSC at 65° C. and subsequent multiple washing in 0.1×SSC at 65° C. for a total of 1 hour; or
(2) hybridizing at 68° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2×SSC and 0.1% SDS at 68° C.
12. An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 1; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1.
13. The isolated promoter of claim 12 inserted into a vector or operably connected to a mobile genetic element.
14. A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 1 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1.
15. A transgenic plant comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ NO: 1 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1.
16. The transgenic plant according to claim 15 , wherein said transgenic plant is Beta vulgaris , and wherein said promoter is active in the root, but not the above ground organs, of Beta vulgaris .
17. The transgenic plant according to claim 15 , wherein said transgenic plant is further characterized by the expression of a transgene exclusively:
a. in at least one of an above-ground or chloroplast containing plant part, or
b. in a below-ground organ,
wherein said expression is obtainable by transformation of a plant cell with said promoter that is operably linked to a transgene.
18. A transgenic seed produced by said transgenic plant of claim 15 .
19. The transgenic plant according to claim 17 , wherein said below-ground organ is a root.
20. The transgenic plant according to claim 17 , wherein said plant belongs to species Beta vulgaris , and wherein said promoter is active in the root, but not the above ground organs, of Beta vulgaris .
21. The transgenic plant according to claim 17 , wherein said transgenic plant is characterized by:
a. amending a carbohydrate metabolism;
b. avoiding a loss of a storage substance;
c. expressing an invertase inhibitor;
d. expressing a fructosyl transferase;
e. expressing a levan sucrase;
f. expressing a gene coding for a transporter protein for a N-compound; or
g. developing a feature that increases at least one of resistance and tolerance towards pathogens.
22. A method of:
(a) amending carbohydrate metabolism,
(b) avoiding loss of storage substance,
(c) increasing at least one of resistance and tolerance towards a pathogen, or
(d) expressing from a transgene:
an invertase inhibitor,
a fructosyl transferase,
a levan sucrase, or
a transporter protein for a N-compound, in a transgenic plant, said method comprising:
a. transforming a plant cell with a promoter, wherein said promoter is operably linked to said transgene, and
b. subsequently regenerating a transgenic plant from said plant cell,
wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
i. SEQ ID NO: 1; and
ii. the polynucleotide sequence complementary to the full length of SEQ ID NO: 1.